Thr328
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Home > Phosphorylation Site Page: > Thr328  -  PKM (human)

Site Information
GkPVICAtQMLESMI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 481749

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 2 , 3 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 1 ) , western blotting ( 1 , 2 )
Disease tissue studied:
brain cancer ( 1 ) , glioblastoma ( 1 ) , glioma ( 1 ) , liver cancer ( 2 ) , hepatocellular carcinoma ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
HSP90A (human) ( 2 )
Putative in vivo kinases:
ATM (human) ( 1 ) , GSK3B (human) ( 2 )
Kinases, in vitro:
GSK3B (human) ( 2 )
Treatments:
ATP ( 1 ) , H2O2 ( 1 ) , KU-55933 ( 1 )

Downstream Regulation
Effects of modification on PKM:
intracellular localization ( 1 ) , protein stabilization ( 2 )
Effects of modification on biological processes:
apoptosis, inhibited ( 2 ) , cell growth, induced ( 2 ) , transcription, induced ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
glioblastoma multiforme ( 1 )

References 

1

Sizemore ST, et al. (2018) Pyruvate kinase M2 regulates homologous recombination-mediated DNA double-strand break repair. Cell Res 28, 1090-1102
30297868   Curated Info

2

Xu Q, et al. (2017) HSP90 promotes cell glycolysis, proliferation and inhibits apoptosis by regulating PKM2 abundance via Thr-328 phosphorylation in hepatocellular carcinoma. Mol Cancer 16, 178
29262861   Curated Info

3

Matsuoka S, et al. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science 316, 1160-6
17525332   Curated Info