Ser477
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Home > Phosphorylation Site Page: > Ser477  -  Akt1 (human)

Site Information
PQFsysAsGtA____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 8086735

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 ) , mutation of modification site ( 4 ) , phospho-antibody ( 1 , 4 ) , western blotting ( 1 , 4 )
Disease tissue studied:
glioblastoma multiforme ( 4 ) , glioma ( 4 ) , breast cancer ( 1 , 3 , 4 ) , breast ductal carcinoma ( 3 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 , 3 ) , gastric cancer ( 6 , 7 ) , gastric carcinoma ( 6 , 7 )
Relevant cell line - cell type - tissue:
breast ( 2 , 3 ) , fibroblast-foreskin ( 4 ) , HCC1428 (breast cell) ( 1 ) , HCC1500 (breast cell) ( 1 ) , HeLa (cervical) ( 4 ) , MCF-7 (breast cell) ( 1 , 4 ) , MEF (fibroblast) ( 4 ) , MKN-45 (gastric) ( 6 , 7 ) , NCI-H3255 (pulmonary) ( 5 ) , T47D (breast cell) ( 1 , 4 ) , T98G (glial) ( 4 )

Upstream Regulation
Putative in vivo kinases:
CDK2 (human) ( 4 )
Kinases, in vitro:
CDK2 (human) ( 4 )
Treatments:
5-methoxypsoralen ( 4 ) , CVT-313 ( 4 ) , insulin ( 4 ) , mimosine ( 4 ) , seliciclib ( 4 )

Downstream Regulation
Effects of modification on Akt1:
enzymatic activity, induced ( 4 ) , molecular association, regulation ( 4 ) , phosphorylation ( 4 )
Effects of modification on biological processes:
apoptosis, inhibited ( 4 ) , carcinogenesis, induced ( 4 ) , cell cycle regulation ( 4 ) , cell growth, induced ( 4 )
Induce interaction with:
Sin1 (human) ( 4 )

References 

1

Jansen VM, et al. (2017) Kinome-Wide RNA Interference Screen Reveals a Role for PDK1 in Acquired Resistance to CDK4/6 Inhibition in ER-Positive Breast Cancer. Cancer Res 77, 2488-2499
28249908   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Liu P, et al. (2014) Cell-cycle-regulated activation of Akt kinase by phosphorylation at its carboxyl terminus. Nature 508, 541-5
24670654   Curated Info

5

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

6

Moritz A (2010) CST Curation Set: 9279; Year: 2010; Biosample/Treatment: cell line, MKN-45/DMSO &'||' Su11274; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

7

Moritz A (2010) CST Curation Set: 9235; Year: 2010; Biosample/Treatment: cell line, MKN-45/calyculin_A & pervanadate; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info