Thr694
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.1.1
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr694  -  Rb-like 2 (human)

Site Information
DsPsDGGtPGRMPPQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449730

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 , 8 ) , immunoprecipitation ( 6 ) , mass spectrometry ( 1 , 2 , 5 , 6 ) , mutation of modification site ( 7 , 8 ) , peptide sequencing ( 8 ) , phosphoamino acid analysis ( 8 ) , phosphopeptide mapping ( 7 , 8 )
Disease tissue studied:
bone cancer ( 8 ) , brain cancer ( 8 ) , glioblastoma ( 8 ) , glioblastoma multiforme ( 8 ) , glioma ( 8 ) , melanoma skin cancer ( 1 )
Relevant cell line - cell type - tissue:
Jurkat (T lymphocyte) ( 2 , 5 ) , Saos-2 (bone cell) ( 8 ) , T lymphocyte-blood ( 8 ) , T98G (glial) ( 8 ) , U2OS (bone cell) [GR (human)] ( 7 , 8 ) , WM239A (melanocyte) ( 1 )

Upstream Regulation
Putative in vivo kinases:
CDK2 (human) ( 8 )

Downstream Regulation
Effects of modification on Rb-like 2:
molecular association, regulation ( 8 )
Effects of modification on biological processes:
cell cycle regulation ( 8 )
Inhibit interaction with:
E2F4 (human) ( 8 )

References 

1

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

2

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

3

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

4

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

5

Zhou J (2011) CST Curation Set: 12497; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Litovchick L, et al. (2011) DYRK1A protein kinase promotes quiescence and senescence through DREAM complex assembly. Genes Dev 25, 801-13
21498570   Curated Info

7

Farkas T, et al. (2002) Distinct phosphorylation events regulate p130- and p107-mediated repression of E2F-4. J Biol Chem 277, 26741-52
12006580   Curated Info

8

Hansen K, et al. (2001) Phosphorylation-dependent and -independent functions of p130 cooperate to evoke a sustained G1 block. EMBO J 20, 422-32
11157749   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2019 Cell Signaling Technology, Inc.