Ser113
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Home > Phosphorylation Site Page: > Ser113  -  BAD (rat)

Site Information
ETRSRHSsYPAGtEE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447860

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 14 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ) , western blotting ( 3 , 4 , 5 , 7 , 8 , 9 , 12 )
Disease tissue studied:
adrenal cancer ( 8 ) , pheochromocytoma ( 8 )
Relevant cell line - cell type - tissue:
'neuron, cerebellar granule'-brain ( 3 , 11 ) , 'neuron, hippocampal'-brain ( 10 ) , adipose tissue ( 2 ) , BHK-21 (fibroblast) ( 9 ) , IEC-6 (epithelial) ( 6 ) , liver ( 2 , 5 ) , oligodendrocyte ( 12 ) , PC-12 (chromaffin) ( 1 , 8 , 14 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 1 ) , Rat1 (fibroblast) ( 13 ) , retina ( 7 ) , retinal ganglion ( 4 ) , stomach ( 2 ) , testis ( 2 )

Upstream Regulation
Regulatory protein:
SNCG (rat) ( 4 )
Kinases, in vitro:
PKACA (rat) ( 11 )
Putative upstream phosphatases:
PPP2CA (rat) ( 6 )
Treatments:
2-AP ( 7 ) , anisomycin ( 7 ) , C5b-9 ( 12 ) , cAMP_analog ( 8 ) , colforsin ( 7 , 9 ) , culturing_of_cells ( 5 ) , eflornithine ( 6 ) , IGF-1 ( 13 ) , ischemia/reperfusion ( 5 ) , LY294002 ( 5 , 7 , 12 ) , MPP+ ( 3 ) , NGF ( 8 ) , okadaic_acid ( 6 ) , PD98059 ( 3 , 5 ) , PDGF ( 1 ) , phorbol_ester ( 8 , 9 ) , PTX ( 12 ) , putrescine ( 6 ) , SB202190 ( 5 ) , SB203580 ( 5 ) , serum ( 9 ) , siRNA ( 4 ) , staurosporine ( 4 ) , TeTx ( 3 ) , TGF-beta ( 10 ) , TNF ( 6 ) , U0126 ( 10 ) , vanadate ( 5 ) , VEGF ( 5 ) , wortmannin ( 3 )

Downstream Regulation
Effects of modification on BAD:
molecular association, regulation ( 3 )
Effects of modification on biological processes:
apoptosis, altered ( 3 , 6 )
Induce interaction with:
14-3-3 beta (rat) ( 3 )
Inhibit interaction with:
Bcl-xL (rat) ( 3 )

References 

1

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Chaïb-Oukadour I, et al. (2009) Tetanus toxin H(C) fragment reduces neuronal MPP+ toxicity. Mol Cell Neurosci 41, 297-303
19344769   Curated Info

4

Surgucheva I, Shestopalov VI, Surguchov A (2008) Effect of gamma-synuclein silencing on apoptotic pathways in retinal ganglion cells. J Biol Chem 283, 36377-85
18936092   Curated Info

5

Ohi N, et al. (2006) Maintenance of Bad phosphorylation prevents apoptosis of rat hepatic sinusoidal endothelial cells in vitro and in vivo. Am J Pathol 168, 1097-106
16565486   Curated Info

6

Ray RM, Bhattacharya S, Johnson LR (2005) Protein phosphatase 2A regulates apoptosis in intestinal epithelial cells. J Biol Chem 280, 31091-100
15994315   Curated Info

7

Campos CB, Bédard PA, Linden R (2003) Selective involvement of the PI3K/PKB/bad pathway in retinal cell death. J Neurobiol 56, 171-7
12838582   Curated Info

8

Affaitati A, et al. (2003) Essential role of A-kinase anchor protein 121 for cAMP signaling to mitochondria. J Biol Chem 278, 4286-94
12427737   Curated Info

9

Yusta B, Estall J, Drucker DJ (2002) Glucagon-like peptide-2 receptor activation engages bad and glycogen synthase kinase-3 in a protein kinase A-dependent manner and prevents apoptosis following inhibition of phosphatidylinositol 3-kinase. J Biol Chem 277, 24896-906
11978789   Curated Info

10

Zhu Y, et al. (2002) Transforming growth factor-beta 1 increases bad phosphorylation and protects neurons against damage. J Neurosci 22, 3898-909
12019309   Curated Info

11

Konishi Y, Lehtinen M, Donovan N, Bonni A (2002) Cdc2 phosphorylation of BAD links the cell cycle to the cell death machinery. Mol Cell 9, 1005-16
12049737   Curated Info

12

Soane L, et al. (2001) C5b-9 terminal complement complex protects oligodendrocytes from death by regulating Bad through phosphatidylinositol 3-kinase/Akt pathway. J Immunol 167, 2305-11
11490019   Curated Info

13

Harada H, et al. (2001) p70S6 kinase signals cell survival as well as growth, inactivating the pro-apoptotic molecule BAD. Proc Natl Acad Sci U S A 98, 9666-70
11493700   Curated Info

14

Datta SR, et al. (1997) Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91, 231-41
9346240   Curated Info