Ser137
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Home > Phosphorylation Site Page: > Ser137  -  BAD (rat)

Site Information
PFRGRsRsAPPNLWA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447862

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 19 ) , immunoprecipitation ( 7 ) , mass spectrometry ( 3 , 4 , 5 ) , mutation of modification site ( 7 , 18 ) , phospho-antibody ( 1 , 2 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ) , western blotting ( 1 , 2 , 6 , 7 , 8 , 9 , 10 , 13 , 15 , 16 , 18 )
Disease tissue studied:
adrenal cancer ( 7 , 13 ) , pheochromocytoma ( 7 , 13 ) , brain cancer ( 18 ) , glioma ( 18 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 4 ) , 'brain, cerebellum' ( 4 ) , 'brain, cerebral cortex' ( 4 ) , 'neuron, cerebellar granule' ( 1 ) , 'neuron, cerebellar granule'-brain ( 6 , 12 , 14 ) , 'neuron, cortical'-brain ( 11 ) , 293A (epithelial) ( 7 ) , adipose tissue ( 4 ) , C2C12 (myoblast) ( 8 ) , C6 (glial) ( 18 ) , HUVEC (endothelial) ( 2 ) , intestine ( 4 ) , kidney ( 4 ) , liver ( 4 , 5 , 9 ) , lung ( 4 ) , myocyte-heart ( 2 , 8 ) , neuron ( 7 ) , oligodendrocyte ( 16 , 18 ) , PC-12 (chromaffin) ( 3 , 7 , 13 , 19 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 3 ) , Rat1 (fibroblast) ( 17 ) , retina ( 10 ) , Schwann ( 15 ) , spleen ( 4 ) , stomach ( 4 ) , testis ( 4 )

Upstream Regulation
Regulatory protein:
MYDGF (rat) ( 2 )
Putative in vivo kinases:
p70S6K (rat) ( 17 )
Kinases, in vitro:
Akt1 (rat) ( 14 ) , p70S6K (human) ( 17 ) , PKACA (rat) ( 14 )
Treatments:
2-AP ( 10 ) , anisomycin ( 10 ) , C5b-9 ( 15 , 16 ) , cAMP_analog ( 13 ) , colforsin ( 10 ) , culturing_of_cells ( 9 ) , haloperidol ( 7 ) , heregulin ( 18 ) , IGF-1 ( 17 ) , ischemia/reperfusion ( 9 ) , LY294002 ( 9 , 10 , 15 , 16 ) , MPP+ ( 6 ) , NGF ( 13 ) , NSC23766 ( 1 ) , PD98059 ( 6 , 9 , 15 ) , PDGF ( 3 ) , phorbol_ester ( 13 ) , pleiotrophin ( 8 ) , PTX ( 16 ) , rapamycin ( 17 ) , SB202190 ( 9 ) , SB203580 ( 9 ) , tauroursodeoxycholic_acid ( 12 ) , TeTx ( 6 ) , vanadate ( 9 ) , VEGF ( 9 ) , wortmannin ( 6 )

Downstream Regulation
Effects of modification on BAD:
activity, induced ( 11 ) , molecular association, regulation ( 6 , 14 )
Effects of modification on biological processes:
apoptosis, altered ( 6 ) , apoptosis, inhibited ( 17 )
Induce interaction with:
14-3-3 beta (rat) ( 6 ) , 14-3-3 zeta (human) ( 14 )
Inhibit interaction with:
Bcl-xL (rat) ( 6 )

References 

1

Stankiewicz TR, et al. (2015) Neuronal Apoptosis Induced by Selective Inhibition of Rac GTPase versus Global Suppression of Rho Family GTPases Is Mediated by Alterations in Distinct Mitogen-activated Protein Kinase Signaling Cascades. J Biol Chem 290, 9363-76
25666619   Curated Info

2

Korf-Klingebiel M, et al. (2015) Myeloid-derived growth factor (C19orf10) mediates cardiac repair following myocardial infarction. Nat Med 21, 140-9
25581518   Curated Info

3

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

4

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

5

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

6

Chaïb-Oukadour I, et al. (2009) Tetanus toxin H(C) fragment reduces neuronal MPP+ toxicity. Mol Cell Neurosci 41, 297-303
19344769   Curated Info

7

Wei Z, et al. (2009) Haloperidol disrupts Akt signalling to reveal a phosphorylation-dependent regulation of pro-apoptotic Bcl-XS function. Cell Signal 21, 161-8
18951975   Curated Info

8

Li J, et al. (2007) The pro-angiogenic cytokine pleiotrophin potentiates cardiomyocyte apoptosis through inhibition of endogenous AKT/PKB activity. J Biol Chem 282, 34984-93
17925408   Curated Info

9

Ohi N, et al. (2006) Maintenance of Bad phosphorylation prevents apoptosis of rat hepatic sinusoidal endothelial cells in vitro and in vivo. Am J Pathol 168, 1097-106
16565486   Curated Info

10

Campos CB, Bédard PA, Linden R (2003) Selective involvement of the PI3K/PKB/bad pathway in retinal cell death. J Neurobiol 56, 171-7
12838582   Curated Info

11

Kuperstein F, Yavin E (2003) Pro-apoptotic signaling in neuronal cells following iron and amyloid beta peptide neurotoxicity. J Neurochem 86, 114-25
12807431   Curated Info

12

Rodrigues CM, et al. (2003) Tauroursodeoxycholic acid reduces apoptosis and protects against neurological injury after acute hemorrhagic stroke in rats. Proc Natl Acad Sci U S A 100, 6087-92
12721362   Curated Info

13

Affaitati A, et al. (2003) Essential role of A-kinase anchor protein 121 for cAMP signaling to mitochondria. J Biol Chem 278, 4286-94
12427737   Curated Info

14

Konishi Y, Lehtinen M, Donovan N, Bonni A (2002) Cdc2 phosphorylation of BAD links the cell cycle to the cell death machinery. Mol Cell 9, 1005-16
12049737   Curated Info

15

Hila S, Soane L, Koski CL (2001) Sublytic C5b-9-stimulated Schwann cell survival through PI 3-kinase-mediated phosphorylation of BAD. Glia 36, 58-67
11571784   Curated Info

16

Soane L, et al. (2001) C5b-9 terminal complement complex protects oligodendrocytes from death by regulating Bad through phosphatidylinositol 3-kinase/Akt pathway. J Immunol 167, 2305-11
11490019   Curated Info

17

Harada H, et al. (2001) p70S6 kinase signals cell survival as well as growth, inactivating the pro-apoptotic molecule BAD. Proc Natl Acad Sci U S A 98, 9666-70
11493700   Curated Info

18

Flores AI, et al. (2000) Akt-mediated survival of oligodendrocytes induced by neuregulins. J Neurosci 20, 7622-30
11027222   Curated Info

19

Datta SR, et al. (1997) Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91, 231-41
9346240   Curated Info