Tyr397
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Home > Phosphorylation Site Page: > Tyr397  -  FAK (chicken)

Site Information
sVsETDDyAEIIDEE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447887

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 , 8 ) , immunoprecipitation ( 6 ) , mass spectrometry ( 1 ) , mutation of modification site ( 3 , 6 , 7 , 8 ) , peptide sequencing ( 8 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 ) , phosphopeptide mapping ( 7 , 8 ) , western blotting ( 2 , 3 , 6 )
Relevant cell line - cell type - tissue:
'stem, embryonic' ( 3 ) , 293 (epithelial) ( 1 , 2 ) , CE (embryonic) ( 7 , 8 ) , CEF ( 4 , 5 , 6 ) , fibroblast ( 2 ) , MEF (fibroblast) ( 2 )

Upstream Regulation
Regulatory protein:
Src (chicken) ( 6 )
Putative in vivo kinases:
FAK (chicken) ( 3 )
Kinases, in vitro:
FAK (chicken) ( 8 )
Treatments:
cell_adhesion ( 2 ) , fibronectin ( 2 , 3 , 5 , 6 ) , heating ( 6 ) , lithium ( 2 ) , low_temperature ( 6 ) , seliciclib ( 2 ) , U0126 ( 2 ) , vanadate ( 7 )

Downstream Regulation
Effects of modification on FAK:
molecular association, regulation ( 6 , 8 )
Induce interaction with:
Src (chicken) ( 6 , 8 )

References 

1

Grigera PR, et al. (2005) FAK phosphorylation sites mapped by mass spectrometry. J Cell Sci 118, 4931-5
16254240   Curated Info

2

Bianchi M, et al. (2005) Regulation of FAK Ser-722 phosphorylation and kinase activity by GSK3 and PP1 during cell spreading and migration. Biochem J 391, 359-70
15975092   Curated Info

3

Leu TH, Maa MC (2002) Tyr-863 phosphorylation enhances focal adhesion kinase autophosphorylation at Tyr-397. Oncogene 21, 6992-7000
12370821   Curated Info

4

Akagi T, Murata K, Shishido T, Hanafusa H (2002) v-Crk activates the phosphoinositide 3-kinase/AKT pathway by utilizing focal adhesion kinase and H-Ras. Mol Cell Biol 22, 7015-23
12242282   Curated Info

5

Datta A, Shi Q, Boettiger DE (2001) Transformation of chicken embryo fibroblasts by v-src uncouples beta1 integrin-mediated outside-in but not inside-out signaling. Mol Cell Biol 21, 7295-306
11585912   Curated Info

6

McLean GW, Fincham VJ, Frame MC (2000) v-Src induces tyrosine phosphorylation of focal adhesion kinase independently of tyrosine 397 and formation of a complex with Src. J Biol Chem 275, 23333-9
10816598   Curated Info

7

Maa MC, Leu TH (1998) Vanadate-dependent FAK activation is accomplished by the sustained FAK Tyr-576/577 phosphorylation. Biochem Biophys Res Commun 251, 344-9
9790958   Curated Info

8

Schaller MD, et al. (1994) Autophosphorylation of the focal adhesion kinase, pp125FAK, directs SH2-dependent binding of pp60src. Mol Cell Biol 14, 1680-8
7509446   Curated Info