Ser369
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.5
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser369  -  KOR-1 (mouse)

Site Information
NtVQDPAsMRDVGGM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449609

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 5 ) , mass spectrometry ( 3 ) , mutation of modification site ( 3 , 6 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ) , western blotting ( 1 , 2 , 6 , 7 )
Disease tissue studied:
neuroblastoma ( 2 , 3 ) , pituitary cancer ( 6 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 8 ) , AtT20 (pituitary cell) ( 6 ) , brain ( 1 ) , Neuro-2a (neuron) ( 2 , 3 ) , neuron-brain ( 4 , 5 ) , spinal cord ( 7 )

Upstream Regulation
Putative in vivo kinases:
GRK3 (human) ( 7 , 8 ) , GRK5 (mouse) ( 2 ) , GRK6 (mouse) ( 2 )
Kinases, in vitro:
GRK3 (human) ( 8 )
Treatments:
42B ( 1 ) , chelerythrine ( 2 ) , etorphine ( 3 ) , GF109203X ( 2 ) , Go_6976 ( 2 ) , MOM-SalB ( 1 ) , nalfurafine ( 1 ) , NBNI ( 7 ) , norBNI ( 3 , 5 ) , phorbol_ester ( 2 ) , PTX ( 2 ) , swim stress ( 4 , 5 ) , U50488 ( 1 , 2 , 3 , 7 , 8 )

Downstream Regulation
Effects of modification on KOR-1:
activity, induced ( 5 ) , intracellular localization ( 3 ) , phosphorylation ( 5 , 6 ) , receptor desensitization, altered ( 8 ) , receptor internalization, induced ( 1 )
Effects of modification on biological processes:
signaling pathway regulation ( 5 )

References 

1

Chen C, et al. (2022) Agonist-Promoted Phosphorylation and Internalization of the Kappa Opioid Receptor in Mouse Brains: Lack of Connection With Conditioned Place Aversion. Front Pharmacol 13, 835809
35652052   Curated Info

2

Chiu YT, et al. (2017) Agonist-Dependent and -Independent κ Opioid Receptor Phosphorylation: Distinct Phosphorylation Patterns and Different Cellular Outcomes. Mol Pharmacol 92, 588-600
28893975   Curated Info

3

Chen C, et al. (2016) Determination of sites of U50,488H-promoted phosphorylation of the mouse κ opioid receptor (KOPR): disconnect between KOPR phosphorylation and internalization. Biochem J 473, 497-508
26635353   Curated Info

4

Lemos JC, et al. (2012) Repeated stress dysregulates κ-opioid receptor signaling in the dorsal raphe through a p38α MAPK-dependent mechanism. J Neurosci 32, 12325-36
22956823   Curated Info

5

Lemos JC, Roth CA, Chavkin C (2011) Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunocolocalization Using Phospho-Selective KOR, p38 MAPK, and K(IR) 3.1 Antibodies. Methods Mol Biol 717, 197-219
21370032   Curated Info

6

Clayton CC, Xu M, Chavkin C (2009) Tyrosine phosphorylation of Kir3 following kappa-opioid receptor activation of p38 MAPK causes heterologous desensitization. J Biol Chem 284, 31872-81
19773548   Curated Info

7

Xu M, et al. (2004) Neuropathic pain activates the endogenous kappa opioid system in mouse spinal cord and induces opioid receptor tolerance. J Neurosci 24, 4576-84
15140929   Curated Info

8

McLaughlin JP, et al. (2004) Prolonged kappa opioid receptor phosphorylation mediated by G-protein receptor kinase underlies sustained analgesic tolerance. J Biol Chem 279, 1810-8
14597630   Curated Info