Thr19
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Home > Phosphorylation Site Page: > Thr19  -  MRLC2 (rat)

Site Information
KKRPQRAtsNVFAMF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448596

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 ) , mass spectrometry (in vitro) ( 5 ) , mutation of modification site ( 4 ) , phospho-antibody ( 6 , 7 , 8 , 9 , 10 ) , western blotting ( 6 , 7 , 8 , 9 )
Disease tissue studied:
adrenal cancer ( 3 , 9 ) , pheochromocytoma ( 3 , 9 )
Relevant cell line - cell type - tissue:
'muscle, smooth' ( 8 ) , adipose tissue ( 2 ) , chromaffin ( 4 ) , heart ( 2 ) , IEC-6 (epithelial) ( 6 ) , intestine ( 2 ) , kidney ( 2 ) , liver ( 2 ) , lung ( 2 ) , neuromuscular junction ( 9 ) , pancreas ( 2 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 1 ) , PC-12 (chromaffin) ( 1 , 3 , 9 ) , REF (fibroblast) ( 10 ) , spleen ( 2 ) , testis ( 2 ) , thymus ( 2 ) , vascular smooth muscle cell ('muscle, smooth') ( 7 )

Upstream Regulation
Regulatory protein:
PIK3C2A (rat) ( 7 )
Kinases, in vitro:
PKACA (cow) ( 5 )
Treatments:
depolarization ( 9 ) , ionomycin ( 7 ) , LY294002 ( 7 ) , microcystin-LR ( 9 ) , norepinephrine ( 7 ) , peptide inhibitor ( 9 ) , peptide_agonist ( 9 ) , staurosporine ( 6 ) , Y27632 ( 8 , 9 )

Downstream Regulation
Effects of modification on MRLC2:
protein conformation ( 4 )
Effects of modification on biological processes:
exocytosis, altered ( 4 )

References 

1

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

4

Neco P, et al. (2008) Myosin II contributes to fusion pore expansion during exocytosis. J Biol Chem 283, 10949-57
18283106   Curated Info

5

Huang SY, et al. (2007) A systematic MS-based approach for identifying in vitro substrates of PKA and PKG in rat uteri. J Proteome Res 6, 2674-84
17564427   Curated Info

6

Ray RM, et al. (2007) Role of myosin regulatory light chain and Rac1 in the migration of polyamine-depleted intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol 292, G983-95
17170026   Curated Info

7

Yoshioka K, Sugimoto N, Takuwa N, Takuwa Y (2007) Essential role for class II phosphoinositide 3-kinase alpha-isoform in Ca2+-induced, Rho- and Rho kinase-dependent regulation of myosin phosphatase and contraction in isolated vascular smooth muscle cells. Mol Pharmacol 71, 912-20
17179444   Curated Info

8

Patel CA, Rattan S (2006) Spontaneously tonic smooth muscle has characteristically higher levels of RhoA/ROK compared with the phasic smooth muscle. Am J Physiol Gastrointest Liver Physiol 291, G830-7
16763289   Curated Info

9

Polo-Parada L, Plattner F, Bose C, Landmesser LT (2005) NCAM 180 acting via a conserved C-terminal domain and MLCK is essential for effective transmission with repetitive stimulation. Neuron 46, 917-31
15953420   Curated Info

10

Murata-Hori M, et al. (2001) HeLa ZIP kinase induces diphosphorylation of myosin II regulatory light chain and reorganization of actin filaments in nonmuscle cells. Oncogene 20, 8175-83
11781833   Curated Info