Ser20
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Home > Phosphorylation Site Page: > Ser20  -  MRLC2 (rat)

Site Information
KRPQRAtsNVFAMFD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448595

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 ) , immunoprecipitation ( 16 ) , mass spectrometry ( 2 , 3 , 4 , 13 ) , mass spectrometry (in vitro) ( 9 ) , mutation of modification site ( 8 ) , phospho-antibody ( 5 , 6 , 7 , 10 , 11 , 12 , 14 , 15 , 16 ) , western blotting ( 5 , 6 , 10 , 11 , 12 , 14 )
Disease tissue studied:
adrenal cancer ( 4 , 14 ) , pheochromocytoma ( 4 , 14 )
Relevant cell line - cell type - tissue:
'kidney, inner medulla' ( 13 ) , 'muscle, smooth' ( 1 , 12 ) , 'muscle, smooth'-thoracic aorta ( 7 ) , adipose tissue ( 3 ) , cerebral artery ( 5 ) , chromaffin ( 8 ) , heart ( 3 ) , IEC-6 (epithelial) ( 10 ) , IMR32 (neural crest) ( 16 ) , intestine ( 3 ) , kidney ( 3 ) , liver ( 3 ) , lung ( 3 ) , neuromuscular junction ( 14 ) , NRK (fibroblast) ( 16 ) , pancreas ( 3 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 2 ) , PC-12 (chromaffin) ( 4 , 14 ) , pulmonary artery ( 6 ) , REF (fibroblast) ( 15 ) , REF52 (fibroblast) ( 16 ) , spleen ( 3 ) , testis ( 3 ) , thymus ( 3 ) , vascular smooth muscle cell ('muscle, smooth') ( 11 )

Upstream Regulation
Regulatory protein:
PIK3C2A (rat) ( 11 )
Kinases, in vitro:
PKACA (cow) ( 9 ) , smMLCK (human) ( 16 )
Treatments:
cGMP_analog ( 5 ) , depolarization ( 14 ) , dinoprost ( 6 ) , hypoxia ( 6 ) , ionomycin ( 11 ) , LY294002 ( 11 ) , microcystin-LR ( 14 ) , norepinephrine ( 11 ) , peptide inhibitor ( 14 ) , peptide_agonist ( 14 ) , phorbol_ester ( 1 ) , staurosporine ( 10 ) , STO-609 ( 7 ) , SU6656 ( 6 ) , U46619 ( 5 ) , vasopressin ( 7 ) , Y27632 ( 6 , 12 , 14 )

Downstream Regulation
Effects of modification on MRLC2:
activity, induced ( 1 ) , intracellular localization ( 16 ) , protein conformation ( 8 )
Effects of modification on biological processes:
exocytosis, altered ( 8 )

References 

1

Dougherty PJ, et al. (2014) PKC activation increases Ca²⁺ sensitivity of permeabilized lymphatic muscle via myosin light chain 20 phosphorylation-dependent and -independent mechanisms. Am J Physiol Heart Circ Physiol 306, H674-83
24414065   Curated Info

2

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

3

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

4

Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

5

Neppl RL, et al. (2009) Thromboxane A2-induced bi-directional regulation of cerebral arterial tone. J Biol Chem 284, 6348-60
19095646   Curated Info

6

Knock GA, et al. (2008) Role of src-family kinases in hypoxic vasoconstriction of rat pulmonary artery. Cardiovasc Res 80, 453-62
18682436   Curated Info

7

Horman S, et al. (2008) AMP-activated protein kinase phosphorylates and desensitizes smooth muscle myosin light chain kinase. J Biol Chem 283, 18505-12
18426792   Curated Info

8

Neco P, et al. (2008) Myosin II contributes to fusion pore expansion during exocytosis. J Biol Chem 283, 10949-57
18283106   Curated Info

9

Huang SY, et al. (2007) A systematic MS-based approach for identifying in vitro substrates of PKA and PKG in rat uteri. J Proteome Res 6, 2674-84
17564427   Curated Info

10

Ray RM, et al. (2007) Role of myosin regulatory light chain and Rac1 in the migration of polyamine-depleted intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol 292, G983-95
17170026   Curated Info

11

Yoshioka K, Sugimoto N, Takuwa N, Takuwa Y (2007) Essential role for class II phosphoinositide 3-kinase alpha-isoform in Ca2+-induced, Rho- and Rho kinase-dependent regulation of myosin phosphatase and contraction in isolated vascular smooth muscle cells. Mol Pharmacol 71, 912-20
17179444   Curated Info

12

Patel CA, Rattan S (2006) Spontaneously tonic smooth muscle has characteristically higher levels of RhoA/ROK compared with the phasic smooth muscle. Am J Physiol Gastrointest Liver Physiol 291, G830-7
16763289   Curated Info

13

Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64
16641100   Curated Info

14

Polo-Parada L, Plattner F, Bose C, Landmesser LT (2005) NCAM 180 acting via a conserved C-terminal domain and MLCK is essential for effective transmission with repetitive stimulation. Neuron 46, 917-31
15953420   Curated Info

15

Murata-Hori M, et al. (2001) HeLa ZIP kinase induces diphosphorylation of myosin II regulatory light chain and reorganization of actin filaments in nonmuscle cells. Oncogene 20, 8175-83
11781833   Curated Info

16

Matsumura F, et al. (1998) Specific localization of serine 19 phosphorylated myosin II during cell locomotion and mitosis of cultured cells. J Cell Biol 140, 119-29
9425160   Curated Info