Ser63
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser63  -  Jun (rat)

Site Information
KNSDLLtsPDVGLLK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447686

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 14 , 15 ) , flow cytometry ( 12 ) , immunoprecipitation ( 14 ) , mutation of modification site ( 5 , 14 , 15 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ) , phosphopeptide mapping ( 15 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 10 , 11 , 12 , 13 )
Disease tissue studied:
adrenal cancer ( 11 ) , pheochromocytoma ( 11 ) , liver cancer ( 10 ) , testicular cancer ( 15 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 3 ) , 'neuron, superior cervical ganglion'-brain ( 9 ) , 'neuron, sympathetic' ( 5 ) , 208F ( 14 ) , 3Y1 (fibroblast) ( 1 ) , adipocyte ( 2 ) , adipocyte-adipose tissue ( 6 ) , F9 (testicular) ( 15 ) , Fr 3T3 (fibroblast) ( 15 ) , H4IIe (hepatic) ( 10 ) , heart ( 8 ) , MEF (fibroblast) ( 15 ) , myocyte ( 13 ) , myocyte-heart ( 4 , 12 ) , PC-12 (chromaffin) ( 11 ) , RIN (epithelial) ( 7 )

Upstream Regulation
Regulatory protein:
HRas (mouse) ( 15 ) , JNK1 (rat) ( 1 ) , JNK2 (rat) ( 1 ) , JNK3 (rat) ( 11 )
Kinases, in vitro:
CDK1 (human) ( 14 ) , CK2A1 (rat) ( 14 )
Treatments:
azaserine ( 7 ) , blood pressure ( 8 ) , bradykinin ( 2 ) , endothelin ( 12 ) , GDNF_withdrawal ( 9 ) , GF109203X ( 12 ) , glucosamine ( 7 ) , glucose ( 7 ) , hypoxia ( 4 ) , hypoxia/reoxygenation ( 4 ) , insulin ( 2 , 6 , 10 ) , ischemia/reperfusion ( 3 ) , JNK_inhibitor_I ( 7 ) , LY294002 ( 10 ) , NGF ( 11 ) , NGF_withdrawal ( 5 , 9 ) , okadaic_acid ( 13 ) , PD98059 ( 12 ) , phenylephrine ( 12 ) , phorbol_ester ( 12 ) , rapamycin ( 10 ) , SB203580 ( 12 ) , serum ( 1 , 14 ) , sorbitol ( 1 , 12 , 13 ) , SP600125 ( 1 , 4 , 6 , 8 , 10 ) , TNF ( 1 ) , U0126 ( 1 , 10 ) , UV ( 1 ) , V-100 ( 4 ) , V-150 ( 4 ) , vanadate ( 3 ) , VX702 ( 4 ) , wortmannin ( 6 )

Downstream Regulation
Effects of modification on Jun:
activity, induced ( 15 ) , protein stabilization ( 12 )
Effects of modification on biological processes:
cell growth, altered ( 15 ) , transcription, altered ( 15 )

References 

1

Xia Y, et al. (2007) c-Jun downregulation by HDAC3-dependent transcriptional repression promotes osmotic stress-induced cell apoptosis. Mol Cell 25, 219-32
17244530   Curated Info

2

Beard KM, Lu H, Ho K, Fantus IG (2006) Bradykinin augments insulin-stimulated glucose transport in rat adipocytes via endothelial nitric oxide synthase-mediated inhibition of Jun NH2-terminal kinase. Diabetes 55, 2678-87
17003331   Curated Info

3

Zhang QG, et al. (2006) Akt inhibits MLK3/JNK3 signaling by inactivating Rac1: a protective mechanism against ischemic brain injury. J Neurochem 98, 1886-98
16831194   Curated Info

4

Shao Z, et al. (2006) c-Jun N-terminal kinases mediate reactivation of Akt and cardiomyocyte survival after hypoxic injury in vitro and in vivo. Circ Res 98, 111-8
16306447   Curated Info

5

Besirli CG, Wagner EF, Johnson EM (2005) The limited role of NH2-terminal c-Jun phosphorylation in neuronal apoptosis: identification of the nuclear pore complex as a potential target of the JNK pathway. J Cell Biol 170, 401-11
16061693   Curated Info

6

Hers I, Tavaré JM (2005) Mechanism of feedback regulation of insulin receptor substrate-1 phosphorylation in primary adipocytes. Biochem J 388, 713-20
15713122   Curated Info

7

Andreozzi F, et al. (2004) Activation of the hexosamine pathway leads to phosphorylation of insulin receptor substrate-1 on Ser307 and Ser612 and impairs the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin insulin biosynthetic pathway in RIN pancreatic beta-cells. Endocrinology 145, 2845-57
15001544   Curated Info

8

Nadruz W, Kobarg CB, Kobarg J, Franchini KG (2004) c-Jun is regulated by combination of enhanced expression and phosphorylation in acute-overloaded rat heart. Am J Physiol Heart Circ Physiol 286, H760-7
14512277   Curated Info

9

Yu LY, et al. (2003) GDNF-deprived sympathetic neurons die via a novel nonmitochondrial pathway. J Cell Biol 163, 987-97
14657232   Curated Info

10

Greene MW, et al. (2003) Modulation of insulin-stimulated degradation of human insulin receptor substrate-1 by Serine 312 phosphorylation. J Biol Chem 278, 8199-211
12510059   Curated Info

11

Waetzig V, Herdegen T (2003) A single c-Jun N-terminal kinase isoform (JNK3-p54) is an effector in both neuronal differentiation and cell death. J Biol Chem 278, 567-72
12401814   Curated Info

12

Clerk A, et al. (2002) Up-regulation of c-jun mRNA in cardiac myocytes requires the extracellular signal-regulated kinase cascade, but c-Jun N-terminal kinases are required for efficient up-regulation of c-Jun protein. Biochem J 368, 101-10
12169099   Curated Info

13

Clerk A, Sugden PH (1997) Cell stress-induced phosphorylation of ATF2 and c-Jun transcription factors in rat ventricular myocytes. Biochem J 325 ( Pt 3), 801-10
9271103   Curated Info

14

Baker SJ, et al. (1992) Jun is phosphorylated by several protein kinases at the same sites that are modified in serum-stimulated fibroblasts. Mol Cell Biol 12, 4694-705
1328860   Curated Info

15

Smeal T, et al. (1991) Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73. Nature 354, 494-6
1749429   Curated Info