Ser676
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.8.0
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser676  -  BUB1B (human)

Site Information
LsPIIEDsREAtHSs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 767909

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 2 ) , immunoassay ( 1 , 2 ) , immunoprecipitation ( 5 ) , mass spectrometry ( 4 , 8 , 9 , 11 ) , mutation of modification site ( 1 , 5 , 7 ) , phospho-antibody ( 1 , 2 , 3 , 7 , 10 , 11 ) , western blotting ( 1 , 2 , 5 , 7 , 10 , 11 )
Disease tissue studied:
cervical cancer ( 2 ) , cervical adenocarcinoma ( 2 )
Relevant cell line - cell type - tissue:
HeLa (cervical) ( 1 , 3 , 4 , 5 , 7 , 9 , 10 , 11 ) , HeLa S3 (cervical) ( 2 ) , HUES-9 ('stem, embryonic') ( 8 )

Upstream Regulation
Regulatory protein:
BUB1 (human) ( 2 ) , CENPE (human) ( 2 )
Putative in vivo kinases:
PLK1 (human) ( 1 , 11 )
Treatments:
AP21967 ( 2 ) , AZ3146 ( 5 ) , BI2536 ( 1 , 5 ) , nocodazole ( 10 ) , okadaic_acid ( 2 ) , SP600125 ( 5 ) , tanespimycin ( 2 ) , taxol ( 10 ) , ZM447439 ( 5 )

Downstream Regulation
Effects of modification on BUB1B:
intracellular localization ( 1 ) , molecular association, regulation ( 1 , 2 , 7 ) , phosphorylation ( 1 , 2 )
Effects of modification on biological processes:
cell cycle regulation ( 1 , 2 , 3 , 6 , 7 ) , cytoskeletal reorganization ( 10 , 11 )
Induce interaction with:
PPP2CA (human) ( 1 , 6 ) , PPP2R5A (human) ( 2 , 7 )

References 

1

Corno A, et al. (2023) A bifunctional kinase-phosphatase module balances mitotic checkpoint strength and kinetochore-microtubule attachment stability. EMBO J, e112630
37712330   Curated Info

2

Gama Braga L, et al. (2020) BUBR1 Pseudokinase Domain Promotes Kinetochore PP2A-B56 Recruitment, Spindle Checkpoint Silencing, and Chromosome Alignment. Cell Rep 33, 108397
33207204   Curated Info

3

Jeffery JM, et al. (2017) FBXO31 protects against genomic instability by capping FOXM1 levels at the G2/M transition. Oncogene 36, 1012-1022
27568981   Curated Info

4

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

5

Tipton AR, et al. (2013) Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex. J Biol Chem 288, 35149-58
24151075   Curated Info

6

Kruse T, et al. (2013) Direct binding between BubR1 and B56-PP2A phosphatase complexes regulate mitotic progression. J Cell Sci 126, 1086-92
23345399   Curated Info

7

Suijkerbuijk SJ, Vleugel M, Teixeira A, Kops GJ (2012) Integration of Kinase and Phosphatase Activities by BUBR1 Ensures Formation of Stable Kinetochore-Microtubule Attachments. Dev Cell 23, 745-55
23079597   Curated Info

8

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

9

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

10

Elowe S, et al. (2010) Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1. J Cell Sci 123, 84-94
20016069   Curated Info

11

Elowe S, et al. (2007) Tension-sensitive Plk1 phosphorylation on BubR1 regulates the stability of kinetochore microtubule interactions. Genes Dev 21, 2205-19
17785528   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2024 Cell Signaling Technology, Inc.