Ser303
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser303  -  p47phox (human)

Site Information
RGAPPRRssIRNAHs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449405

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 , 7 ) , immunoprecipitation ( 3 ) , mutation of modification site ( 1 , 3 , 5 , 6 ) , peptide sequencing ( 7 ) , phospho-antibody ( 2 , 3 ) , phosphoamino acid analysis ( 7 ) , phosphopeptide mapping ( 6 , 7 ) , western blotting ( 2 , 3 )
Disease tissue studied:
leukemia ( 5 ) , chronic myelogenous leukemia ( 5 )
Relevant cell line - cell type - tissue:
B lymphocyte-spleen ( 6 ) , COS7 (fibroblast) ( 3 ) , K562 (erythroid) ( 5 ) , microvessel endothelial ( 1 ) , neutrophil ( 6 , 7 ) , neutrophil-blood ( 2 ) , SVEC (endothelial) ( 3 )

Upstream Regulation
Kinases, in vitro:
PKCA (human) ( 4 , 6 ) , PKCB iso2 (human) ( 4 ) , PKCD (human) ( 4 )
Treatments:
CL097 ( 2 ) , fMLP ( 7 ) , phorbol_ester ( 7 )

Downstream Regulation
Effects of modification on p47phox:
activity, induced ( 4 ) , enzymatic activity, induced ( 5 ) , molecular association, regulation ( 4 , 5 )
Induce interaction with:
CYBA (human) ( 4 , 5 )

References 

1

Meijles DN, Fan LM, Howlin BJ, Li JM (2014) Molecular Insights of p47phox Phosphorylation Dynamics in the Regulation of NADPH Oxidase Activation and Superoxide Production. J Biol Chem 289, 22759-70
24970888   Curated Info

2

Makni-Maalej K, et al. (2012) The TLR7/8 Agonist CL097 Primes N-Formyl-Methionyl-Leucyl-Phenylalanine-Stimulated NADPH Oxidase Activation in Human Neutrophils: Critical Role of p47phox Phosphorylation and the Proline Isomerase Pin1. J Immunol 189, 4657-65
23002436   Curated Info

3

Teng L, Fan LM, Meijles D, Li JM (2012) Divergent Effects of p47phox Phosphorylation at S303-4 or S379 on Tumor Necrosis Factor-α Signaling via TRAF4 and MAPK in Endothelial Cells. Arterioscler Thromb Vasc Biol 32, 1488-96
22460559   Curated Info

4

Fontayne A, Dang PM, Gougerot-Pocidalo MA, El-Benna J (2002) Phosphorylation of p47phox sites by PKC alpha, beta II, delta, and zeta: effect on binding to p22phox and on NADPH oxidase activation. Biochemistry 41, 7743-50
12056906   Curated Info

5

Ago T, Nunoi H, Ito T, Sumimoto H (1999) Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating the oxidase. J Biol Chem 274, 33644-53
10559253   Curated Info

6

El Benna J, Faust RP, Johnson JL, Babior BM (1996) Phosphorylation of the respiratory burst oxidase subunit p47phox as determined by two-dimensional phosphopeptide mapping. Phosphorylation by protein kinase C, protein kinase A, and a mitogen-activated protein kinase. J Biol Chem 271, 6374-8
8626435   Curated Info

7

el Benna J, Faust LP, Babior BM (1994) The phosphorylation of the respiratory burst oxidase component p47phox during neutrophil activation. Phosphorylation of sites recognized by protein kinase C and by proline-directed kinases. J Biol Chem 269, 23431-6
8089108   Curated Info