Ser724
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Home > Phosphorylation Site Page: > Ser724  -  IRE1 (mouse)

Site Information
KLAVGRHsFSRRsGV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 7161100

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 11 ) , mutation of modification site ( 4 , 9 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 7 , 9 , 10 , 11 ) , western blotting ( 1 , 3 , 4 , 5 , 7 , 9 , 10 , 11 )
Disease tissue studied:
pancreatic cancer ( 11 ) , pancreatic carcinoma ( 11 )
Relevant cell line - cell type - tissue:
'brain, hypothalamus' ( 10 ) , 293 (epithelial) ( 10 ) , 3T3-L1 (fibroblast) ( 5 ) , beta-pancreas ( 11 ) , COS (fibroblast) ( 11 ) , HEK293T (epithelial) ( 1 , 7 ) , hepatocyte-liver ( 4 , 9 ) , INS-1 (pancreatic) ( 11 ) , liver ( 3 , 9 ) , MEF (fibroblast) ( 4 )

Upstream Regulation
Regulatory protein:
Fyn (mouse) ( 7 ) , VMP1 (human) ( 1 )
Putative in vivo kinases:
Akt1 (human) ( 3 ) , PKACA (human) ( 9 )
Kinases, in vitro:
Akt1 (human) ( 3 ) , PKACA (mouse) ( 9 )
Treatments:
cAMP_analog ( 9 ) , fasting ( 9 ) , glucagon ( 9 ) , glucose ( 11 ) , H-89 ( 9 ) , high-fat diet ( 10 ) , insulin ( 3 ) , KirA6 ( 4 , 5 ) , LY294002 ( 3 ) , NKH_477 ( 9 ) , obesity-inducing diet ( 9 ) , peptidoglycan ( 5 ) , racepinefrine ( 9 ) , rapamycin ( 7 ) , refeeding ( 3 ) , SH-5 ( 3 ) , siRNA ( 9 ) , thapsigargin ( 4 , 5 , 7 , 9 ) , tunicamycin ( 4 , 11 ) , U73122 ( 9 )

Downstream Regulation
Effects of modification on IRE1:
enzymatic activity, induced ( 4 , 5 ) , phosphorylation ( 4 )
Effects of modification on biological processes:
apoptosis, induced ( 7 ) , RNA splicing, altered ( 9 ) , RNA splicing, induced ( 3 , 4 ) , transcription, altered ( 4 ) , transcription, induced ( 3 , 5 , 9 )

References 

1

Li T, et al. (2023) VMP1 affects endoplasmic reticulum stress sensitivity via differential modulation of the three unfolded protein response arms. Cell Rep 42, 112209
36870060   Curated Info

2

Li M, et al. (2023) Semaphorin 3A Inhibits Endoplasmic Reticulum Stress Induced by High Glucose in Müller Cells. Curr Eye Res 48, 70-79
36271834   Curated Info

3

Peng J, et al. (2022) Activation of the canonical ER Stress IRE1-XBP1 Pathway by Insulin Regulates Glucose and Lipid Metabolism. J Biol Chem, 102283
35863429   Curated Info

4

Li Y, et al. (2022) Phosphorylation at Ser of the ER stress sensor IRE1α governs its activation state and limits ER stress-induced hepatosteatosis. J Biol Chem 298, 101997
35500653   Curated Info

5

Foley KP, et al. (2021) Inflammation promotes adipocyte lipolysis via IRE1 kinase. J Biol Chem, 100440
33610548   Curated Info

6

Puckett DL, et al. (2020) Zyflamend induces apoptosis in pancreatic cancer cells via modulation of the JNK pathway. Cell Commun Signal
32795297   Curated Info

7

Wang Y, Yamada E, Zong H, Pessin JE (2015) Fyn Activation of mTORC1 Stimulates the IRE1α-JNK Pathway, Leading to Cell Death. J Biol Chem 290, 24772-83
26306048   Curated Info

8

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

9

Mao T, et al. (2011) PKA phosphorylation couples hepatic inositol-requiring enzyme 1alpha to glucagon signaling in glucose metabolism. Proc Natl Acad Sci U S A 108, 15852-7
21911379   Curated Info

10

Ozcan L, et al. (2009) Endoplasmic reticulum stress plays a central role in development of leptin resistance. Cell Metab 9, 35-51
19117545   Curated Info

11

Lipson KL, et al. (2006) Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1. Cell Metab 4, 245-54
16950141   Curated Info