Ser419

Site Information
rIEEELGskAkFAGR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 7104866

In vivo Characterization
Methods used to characterize site in vivo:	2D analysis ( 13 ) , mass spectrometry ( 1 , 2 , 5 , 6 , 7 , 8 , 10 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ) , phospho-antibody ( 4 )
Disease tissue studied:	breast cancer ( 7 ) , breast ductal carcinoma ( 7 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 , 7 ) , cervical cancer ( 19 ) , cervical adenocarcinoma ( 19 ) , hepatocellular carcinoma, surrounding tissue ( 18 ) , lung cancer ( 8 , 10 ) , non-small cell lung cancer ( 10 ) , non-small cell lung adenocarcinoma ( 8 ) , pancreatic cancer ( 13 , 17 ) , pancreatic carcinoma ( 13 , 17 ) , pancreatic ductal adenocarcinoma ( 4 ) , melanoma skin cancer ( 5 )
Relevant cell line - cell type - tissue:	'muscle, skeletal' ( 12 , 21 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 16 ) , breast ( 2 , 7 ) , CFPAC-1 (pancreatic) ( 13 , 17 ) , HeLa (cervical) ( 6 , 15 ) , HeLa S3 (cervical) ( 19 ) , hepatocyte-liver ( 18 ) , HOP62 (pulmonary) ( 10 ) , HUES-9 ('stem, embryonic') ( 14 ) , K562 (erythroid) ( 20 ) , lung ( 8 ) , NCI-H322 (pulmonary) ( 10 ) , pancreas ( 13 ) , pancreatic ( 17 ) , T lymphocyte-blood ( 4 ) , Vero E6-S ('epithelial, kidney') ( 1 ) , WM239A (melanocyte) ( 5 )

Upstream Regulation
Treatments:	MLN8054 ( 15 )

Downstream Regulation
Effects of modification on biological processes:	cell growth, induced ( 4 ) , signaling pathway regulation ( 4 )

Disease / Diagnostics Relevance
Relevant diseases:	pancreatic carcinoma ( 13 )

References
1	Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell 182 32645325 Curated Info
2	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
3	Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610 27184836 Curated Info
4	Capello M, et al. (2015) Phosphorylated alpha-enolase induces autoantibodies in HLA-DR8 pancreatic cancer patients and triggers HLA-DR8 restricted T-cell activation. Immunol Lett 167, 11-6 26096821 Curated Info
5	Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615 25850435 Curated Info
6	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
7	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
8	Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96 23911959 Curated Info
9	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
10	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
11	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
12	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
13	Tomaino B, et al. (2011) Circulating autoantibodies to phosphorylated α-enolase are a hallmark of pancreatic cancer. J Proteome Res 10, 105-12 20455595 Curated Info
14	Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3 21406692 Curated Info
15	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
16	Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53 20363803 Curated Info
17	Zhou W, et al. (2010) Mass spectrometry analysis of the post-translational modifications of alpha-enolase from pancreatic ductal adenocarcinoma cells. J Proteome Res 9, 2929-36 20433201 Curated Info
18	Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9 20166139 Curated Info
19	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
20	Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808 19651622 Curated Info
21	Højlund K, et al. (2009) In vivo phosphoproteome of human skeletal muscle revealed by phosphopeptide enrichment and HPLC-ESI-MS/MS. J Proteome Res 8, 4954-65 19764811 Curated Info