Ser1169
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Home > Phosphorylation Site Page: > Ser1169  -  WNK4 (mouse)

Site Information
SCRQRRLsKGsFPTS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 477191

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 6 ) , mass spectrometry ( 1 , 2 , 3 , 4 ) , mutation of modification site ( 5 , 6 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 , 3 ) , COS7 (fibroblast) ( 1 ) , HEK293T (epithelial) ( 1 , 6 ) , oocyte ( 5 ) , oocyte [CPEB (mouse)] ( 6 ) , testis ( 4 )

Upstream Regulation
Putative in vivo kinases:
SGK1 (human) ( 6 )
Kinases, in vitro:
SGK1 (human) ( 5 , 6 )
Treatments:
angiotensin_2 ( 1 ) , bisindolylmaleimide ( 1 ) , colforsin ( 1 ) , H-89 ( 1 ) , insulin ( 3 ) , MK-2206 ( 3 ) , phorbol_ester ( 1 )

Downstream Regulation
Effects of modification on WNK4:
activity, inhibited ( 6 )

References 

1

Castañeda-Bueno M, et al. (2017) Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4. Proc Natl Acad Sci U S A 114, E879-E886
28096417   Curated Info

2

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

3

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

4

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

5

Rozansky DJ, et al. (2009) Aldosterone mediates activation of the thiazide-sensitive Na-Cl cotransporter through an SGK1 and WNK4 signaling pathway. J Clin Invest 119, 2601-12
19690383   Curated Info

6

Ring AM, et al. (2007) An SGK1 site in WNK4 regulates Na+ channel and K+ channel activity and has implications for aldosterone signaling and K+ homeostasis. Proc Natl Acad Sci U S A 104, 4025-9
17360471   Curated Info

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