Ser659

Site Information
TLPGKAEsVVsLTSQ SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 449331

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 2 ) , mass spectrometry ( 4 ) , mutation of modification site ( 1 , 2 , 3 ) , phospho-antibody ( 1 , 3 ) , western blotting ( 1 , 2 , 3 )
Disease tissue studied:	familial advanced sleep phase syndrome ( 3 , 4 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 1 , 2 , 4 ) , MEF (fibroblast) ( 3 )

Downstream Regulation
Effects of modification on PER2:	intracellular localization ( 4 ) , protein degradation ( 3 ) , protein stabilization ( 4 )

References
1	Narasimamurthy R, et al. (2018) CK1δ/ε protein kinase primes the PER2 circadian phosphoswitch. Proc Natl Acad Sci U S A 115, 5986-5991 29784789 Curated Info
2	Eng GWL, Edison, Virshup DM (2017) Site-specific phosphorylation of casein kinase 1 δ (CK1δ) regulates its activity towards the circadian regulator PER2. PLoS One 12, e0177834 28545154 Curated Info
3	Zhou M, et al. (2015) A Period2 Phosphoswitch Regulates and Temperature Compensates Circadian Period. Mol Cell 60, 77-88 26431025 Curated Info
4	Vanselow K, et al. (2006) Differential effects of PER2 phosphorylation: molecular basis for the human familial advanced sleep phase syndrome (FASPS). Genes Dev 20, 2660-72 16983144 Curated Info