Ser65
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Home > Phosphorylation Site Page: > Ser65  -  BIM (rat)

Site Information
GPLAPPAsPGPFATR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449330

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 3 , 5 ) , electrophoretic mobility shift ( 1 , 2 , 3 , 4 ) , mutation of modification site ( 1 , 2 , 3 , 4 , 5 ) , phospho-antibody ( 1 , 4 ) , western blotting ( 1 , 4 )
Disease tissue studied:
adrenal cancer ( 1 , 4 ) , pheochromocytoma ( 1 , 4 )
Relevant cell line - cell type - tissue:
'neuron, cerebellar granule'-brain ( 2 , 4 ) , 293 (epithelial) ( 1 , 3 , 5 ) , HEK293T (epithelial) ( 2 , 4 ) , PC-12 (chromaffin) ( 1 , 4 )

Upstream Regulation
Regulatory protein:
MEKK1 (human) ( 4 ) , MKK3 (rat) ( 1 ) , NGFR (human) ( 4 )
Putative in vivo kinases:
ERK1 (human) ( 3 , 5 ) , ERK2 (human) ( 3 , 5 ) , JNK1 (human) ( 2 , 4 ) , P38A (human) ( 1 , 4 )
Kinases, in vitro:
ERK1 (human) ( 5 ) , JNK1 (human) ( 4 ) , P38A (human) ( 1 )
Treatments:
arsenite ( 1 ) , osmotic_stress ( 4 ) , PD184352 ( 5 ) , SB203580 ( 1 , 4 ) , serum ( 3 , 5 ) , SP600125 ( 4 ) , U0126 ( 3 )

Downstream Regulation
Effects of modification on BIM:
activity, induced ( 4 ) , molecular association, regulation ( 2 ) , protein degradation ( 3 , 5 )
Effects of modification on biological processes:
apoptosis, induced ( 1 , 4 )
Induce interaction with:
PIN1 (human) ( 2 )

References 

1

Cai B, et al. (2006) p38 MAP kinase mediates apoptosis through phosphorylation of BimEL at Ser-65. J Biol Chem 281, 25215-22
16818494   Curated Info

2

Becker EB, Bonni A (2006) Pin1 mediates neural-specific activation of the mitochondrial apoptotic machinery. Neuron 49, 655-62
16504941   Curated Info

3

Ley R, Hadfield K, Howes E, Cook SJ (2005) Identification of a DEF-type docking domain for extracellular signal-regulated kinases 1/2 that directs phosphorylation and turnover of the BH3-only protein BimEL. J Biol Chem 280, 17657-63
15728578   Curated Info

4

Becker EB, et al. (2004) Characterization of the c-Jun N-terminal kinase-BimEL signaling pathway in neuronal apoptosis. J Neurosci 24, 8762-70
15470142   Curated Info

5

Ley R, et al. (2004) Extracellular signal-regulated kinases 1/2 are serum-stimulated "Bim(EL) kinases" that bind to the BH3-only protein Bim(EL) causing its phosphorylation and turnover. J Biol Chem 279, 8837-47
14681225   Curated Info