Ser65
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Home > Phosphorylation Site Page: > Ser65  -  BIM (mouse)

Site Information
GPLAPPAsPGPFATR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449330

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 4 , 5 ) , flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 ) , mutation of modification site ( 1 , 4 , 5 , 6 , 7 ) , phospho-antibody ( 4 , 6 , 7 ) , western blotting ( 1 , 4 , 6 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 ) , 'neuron, cerebellar granule'-brain ( 7 ) , 293 (epithelial) ( 4 , 7 ) , epithelial-mammary gland ( 1 ) , ES-J1 (stem) ( 3 ) , FL5.12 (lymphoid) [BIM (mouse)] ( 6 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 5 ) , kidney ( 1 ) , MEF (fibroblast) ( 4 ) , RAW 264 (macrophage) ( 5 )

Upstream Regulation
Putative in vivo kinases:
ERK1 (mouse) ( 5 , 6 ) , ERK2 (mouse) ( 5 , 6 )
Kinases, in vitro:
JNK1 (human) ( 7 ) , JNK2 (human) ( 7 ) , JNK3 (human) ( 7 )
Treatments:
Boc-D-FMK ( 7 ) , calyculin_A ( 5 ) , CEP1347 ( 7 ) , IL-3 ( 6 ) , insulin ( 2 ) , LPS ( 5 ) , LY294002 ( 6 ) , NGF_withdrawal ( 7 ) , PD98059 ( 6 ) , SB203580 ( 6 ) , serum ( 4 ) , SP600125 ( 6 , 7 ) , U0126 ( 6 ) , UV ( 4 )

Downstream Regulation
Effects of modification on BIM:
molecular association, regulation ( 6 ) , protein degradation ( 4 , 5 )
Effects of modification on biological processes:
apoptosis, altered ( 6 ) , apoptosis, induced ( 7 ) , apoptosis, inhibited ( 4 , 5 )
Inhibit interaction with:
BAX (mouse) ( 6 )

References 

1

Girnius N, Davis RJ (2017) JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins. Cell Rep 21, 1910-1921
29141222   Curated Info

2

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

3

Zhou J (2010) CST Curation Set: 9676; Year: 2010; Biosample/Treatment: cell line, ES J1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

4

Hübner A, Barrett T, Flavell RA, Davis RJ (2008) Multisite phosphorylation regulates Bim stability and apoptotic activity. Mol Cell 30, 415-25
18498746   Curated Info

5

Häcker G, Suttner K, Harada H, Kirschnek S (2006) TLR-dependent Bim phosphorylation in macrophages is mediated by ERK and is connected to proteasomal degradation of the protein. Int Immunol 18, 1749-57
17105963   Curated Info

6

Harada H, Quearry B, Ruiz-Vela A, Korsmeyer SJ (2004) Survival factor-induced extracellular signal-regulated kinase phosphorylates BIM, inhibiting its association with BAX and proapoptotic activity. Proc Natl Acad Sci U S A 101, 15313-7
15486085   Curated Info

7

Putcha GV, et al. (2003) JNK-mediated BIM phosphorylation potentiates BAX-dependent apoptosis. Neuron 38, 899-914
12818176   Curated Info