Thr53
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr53  -  DAT (rat)

Site Information
TLINPPQtPVEAQER   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 6843600

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 ) , immunoassay ( 2 ) , immunoprecipitation ( 2 , 3 ) , mass spectrometry ( 2 ) , mutation of modification site ( 1 , 2 , 3 ) , phospho-antibody ( 1 , 2 ) , phosphoamino acid analysis ( 3 ) , western blotting ( 1 , 2 )
Relevant cell line - cell type - tissue:
'brain, striatum' ( 1 , 2 ) , LLC-PK1 (renal) ( 1 , 2 , 3 ) , neuron:synaptosome ( 1 ) , tsA201 (epithelial) ( 2 )

Upstream Regulation
Regulatory protein:
ERK1 (rat) ( 2 )
Kinases, in vitro:
ERK1 (human) ( 3 )
Treatments:
benzatropine ( 1 ) , cocaine ( 1 ) , dopamine ( 1 ) , levamfetamine ( 1 ) , metamfetamine ( 1 ) , okadaic_acid ( 1 , 2 ) , phorbol_ester ( 2 , 3 )

Downstream Regulation
Effects of modification on DAT:
activity, induced ( 2 )

References 

1

Challasivakanaka S, et al. (2017) Dopamine transporter phosphorylation site threonine 53 is stimulated by amphetamines and regulates dopamine transport, efflux, and cocaine analog binding. J Biol Chem 292, 19066-19075
28939767   Curated Info

2

Foster JD, et al. (2012) Dopamine Transporter Phosphorylation Site Threonine 53 Regulates Substrate Reuptake and Amphetamine-stimulated Efflux. J Biol Chem 287, 29702-12
22722938   Curated Info

3

Gorentla BK, Moritz AE, Foster JD, Vaughan RA (2009) Proline-directed phosphorylation of the dopamine transporter N-terminal domain. Biochemistry 48, 1067-76
19146407   Curated Info