Thr45
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Home > Phosphorylation Site Page: > Thr45  -  PKM (human)

Site Information
PPItARNtGIICtIG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 476071

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 ) , mutation of modification site ( 2 ) , phospho-antibody ( 2 ) , western blotting ( 2 )
Disease tissue studied:
brain cancer ( 2 ) , glioblastoma ( 2 ) , glioma ( 2 ) , lung cancer ( 2 , 3 , 5 ) , non-small cell lung cancer ( 2 , 5 ) , non-small cell lung adenocarcinoma ( 2 , 3 , 5 ) , pancreatic cancer ( 2 ) , pancreatic carcinoma ( 2 ) , melanoma skin cancer ( 2 )
Relevant cell line - cell type - tissue:
A549 (pulmonary) ( 2 ) , BxPC-3 (pancreatic) ( 2 ) , GSCs (glial) ( 2 ) , HEK293T (epithelial) ( 6 ) , HeLa (cervical) ( 1 , 2 , 4 ) , HS294T (melanocyte) ( 2 ) , lung ( 3 ) , MEF (fibroblast) ( 2 ) , NCI-H1975 (pulmonary) ( 5 ) , U87MG (glial) ( 2 )

Upstream Regulation
Regulatory protein:
BRAF (human) ( 2 ) , EGFR (human) ( 2 ) , KRas (human) ( 2 )
Putative in vivo kinases:
AurB (human) ( 2 )
Kinases, in vitro:
AurB (human) ( 2 )
Treatments:
AZD1152 ( 2 ) , doxycycline ( 2 ) , ML-7 ( 2 ) , Y27632 ( 2 )

Downstream Regulation
Effects of modification on PKM:
intracellular localization ( 2 ) , molecular association, regulation ( 2 ) , phosphorylation ( 2 )
Effects of modification on biological processes:
carcinogenesis, induced ( 2 ) , cell cycle regulation ( 2 ) , cell growth, induced ( 2 )
Induce interaction with:
MRLC2V (human) ( 2 )

References 

1

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

2

Jiang Y, et al. (2014) PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells. Nat Commun 5, 5566
25412762   Curated Info

3

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

4

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

5

Moritz A (2009) CST Curation Set: 7293; Year: 2009; Biosample/Treatment: cell line, NCI-H1975/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Molina H, et al. (2007) Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry. Proc Natl Acad Sci U S A 104, 2199-204
17287340   Curated Info