Ser282
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Home > Phosphorylation Site Page: > Ser282  -  GJA1 (rat)

Site Information
TAPLsPMsPPGYkLV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448854

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 11 , 12 ) , electrophoretic mobility shift ( 9 ) , immunoprecipitation ( 1 , 2 , 6 ) , mutation of modification site ( 1 , 3 , 6 , 11 ) , peptide sequencing ( 2 ) , phospho-antibody ( 9 , 10 ) , phosphoamino acid analysis ( 11 ) , phosphopeptide mapping ( 12 ) , western blotting ( 1 , 2 , 6 , 9 )
Disease tissue studied:
pancreatic cancer ( 6 ) , pancreatic carcinoma ( 6 )
Relevant cell line - cell type - tissue:
BxPC-3 (pancreatic) ( 6 ) , HeLa (cervical) ( 1 ) , MDCK (epithelial) ( 1 ) , NRK (fibroblast) ( 2 ) , T51B (epithelial) ( 12 ) , WB-F344 (epithelial) ( 9 , 10 )

Upstream Regulation
Regulatory protein:
Tyk2 (human) ( 2 )
Putative in vivo kinases:
ERK1 (rat) ( 9 ) , ERK2 (rat) ( 9 )
Kinases, in vitro:
ERK1 (rat) ( 12 ) , ERK2 (mouse) ( 11 )
Treatments:
adriamycin ( 9 ) , angiotensin_2 ( 2 ) , ATP ( 4 ) , BQ ( 10 ) , DEM ( 10 ) , DMNQ ( 10 ) , EGF ( 11 ) , IP3/BM ( 4 ) , LPA ( 11 ) , menadione ( 9 , 10 ) , PD98059 ( 11 ) , phorbol_ester ( 4 ) , vanadate ( 11 )

Downstream Regulation
Effects of modification on GJA1:
activity, inhibited ( 11 ) , molecular association, regulation ( 3 )
Induce interaction with:
GJA1 (rat) ( 3 )

Disease / Diagnostics Relevance
Relevant diseases:
hypertrophic cardiomyopathy ( 7 )

References 

1

Thévenin AF, et al. (2017) Phosphorylation regulates connexin43/ZO-1 binding and release, an important step in gap junction turnover. Mol Biol Cell 28, 3595-3608
29021339   Curated Info

2

Li H, et al. (2016) Regulation of Connexin43 Function and Expression by Tyrosine Kinase 2. J Biol Chem 291, 15867-80
27235399   Curated Info

3

Spagnol G, et al. (2016) Structural Studies of the Nedd4 WW Domains and Their Selectivity for the Connexin43 (Cx43) Carboxyl Terminus. J Biol Chem 291, 7637-50
26841867   Curated Info

4

Kang M, et al. (2014) Cx43 phosphorylation on S279/282 and intercellular communication are regulated by IP3/IP3 receptor signaling. Cell Commun Signal 12, 58
25262337   Curated Info

5

Li H, et al. (2014) TC-PTP directly interacts with connexin43 to regulate gap junction intercellular communication. J Cell Sci 127, 3269-79
24849651   Curated Info

6

Johnson KE, et al. (2013) Phosphorylation on Ser-279 and Ser-282 of connexin43 regulates endocytosis and gap junction assembly in pancreatic cancer cells. Mol Biol Cell 24, 715-33
23363606   Curated Info

7

Curcio A, et al. (2013) MicroRNA-1 downregulation increases connexin 43 displacement and induces ventricular tachyarrhythmias in rodent hypertrophic hearts. PLoS One 8, e70158
23922949   Curated Info

8

Mitra SS, Xu J, Nicholson BJ (2012) Coregulation of Multiple Signaling Mechanisms in pp60v-Src-Induced Closure of Cx43 Gap Junction Channels. J Membr Biol 245, 495-506
22965738   Curated Info

9

Abdelmohsen K, et al. (2005) Doxorubicin induces EGF receptor-dependent downregulation of gap junctional intercellular communication in rat liver epithelial cells. Biol Chem 386, 217-23
15843167   Curated Info

10

Abdelmohsen K, et al. (2003) Epidermal growth factor receptor is a common mediator of quinone-induced signaling leading to phosphorylation of connexin-43: role of glutathione and tyrosine phosphatases. J Biol Chem 278, 38360-7
12874275   Curated Info

11

Warn-Cramer BJ, Cottrell GT, Burt JM, Lau AF (1998) Regulation of connexin-43 gap junctional intercellular communication by mitogen-activated protein kinase. J Biol Chem 273, 9188-96
9535909   Curated Info

12

Warn-Cramer BJ, et al. (1996) Characterization of the mitogen-activated protein kinase phosphorylation sites on the connexin-43 gap junction protein. J Biol Chem 271, 3779-86
8631994   Curated Info