Tyr265
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Home > Phosphorylation Site Page: > Tyr265  -  GJA1 (rat)

Site Information
kDCGsPkyAyFNGCS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447815

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 12 ) , immunoprecipitation ( 3 , 4 , 5 ) , mass spectrometry ( 9 ) , mutation of modification site ( 3 , 8 , 10 , 11 , 12 , 13 ) , peptide sequencing ( 4 ) , phospho-antibody ( 2 , 6 , 13 ) , phosphopeptide mapping ( 12 ) , western blotting ( 2 , 3 , 4 , 6 )
Disease tissue studied:
brain cancer ( 3 , 8 ) , glioma ( 3 , 8 )
Relevant cell line - cell type - tissue:
brain ( 5 , 9 ) , C6 (glial) ( 3 , 8 ) , COS (fibroblast) ( 13 ) , E.coli (bacterial) ( 5 ) , fibroblast [GJA1 (mouse)] ( 10 ) , LA-25 (fibroblast) ( 6 ) , MEF (fibroblast) ( 11 ) , NRK (fibroblast) ( 4 ) , Rat1 (fibroblast) ( 13 ) , Vero (epithelial) ( 5 ) , VSMC-aorta ( 2 )

Upstream Regulation
Putative in vivo kinases:
Src (rat) ( 6 , 13 ) , Tyk2 (human) ( 4 )
Kinases, in vitro:
Src (human) ( 12 ) , Src (rat) ( 13 ) , Tyk2 (human) ( 4 )
Putative upstream phosphatases:
PTPN2 (human) ( 6 )
Phosphatases, in vitro:
PTPN2 (human) ( 6 )
Treatments:
angiotensin_2 ( 4 ) , ouabain ( 2 )

Downstream Regulation
Effects of modification on GJA1:
activity, induced ( 3 ) , activity, inhibited ( 10 , 11 ) , intracellular localization ( 6 ) , molecular association, regulation ( 5 , 6 , 13 )
Effects of modification on biological processes:
carcinogenesis, altered ( 8 ) , cell adhesion, altered ( 13 ) , cell growth, altered ( 8 , 11 )
Induce interaction with:
DBN1 (human) ( 5 ) , PTPN2 (human) ( 6 ) , Src (rat) ( 13 )

References 

1

Spagnol G, et al. (2018) Connexin43 Carboxyl-Terminal Domain Directly Interacts with β-Catenin. Int J Mol Sci 19
29882937   Curated Info

2

Hangaard L, et al. (2017) Na-K-ATPase regulates intercellular communication in the vascular wall via cSrc kinase-dependent connexin43 phosphorylation. Am J Physiol Cell Physiol 312, C385-C397
28122732   Curated Info

3

González-Sánchez A, et al. (2016) Connexin43 recruits PTEN and Csk to inhibit c-Src activity in glioma cells and astrocytes. Oncotarget 7, 49819-49833
27391443   Curated Info

4

Li H, et al. (2016) Regulation of Connexin43 Function and Expression by Tyrosine Kinase 2. J Biol Chem 291, 15867-80
27235399   Curated Info

5

Ambrosi C, et al. (2016) Connexin43 Forms Supramolecular Complexes through Non-Overlapping Binding Sites for Drebrin, Tubulin, and ZO-1. PLoS One 11, e0157073
27280719   Curated Info

6

Li H, et al. (2014) TC-PTP directly interacts with connexin43 to regulate gap junction intercellular communication. J Cell Sci 127, 3269-79
24849651   Curated Info

7

Mitra SS, Xu J, Nicholson BJ (2012) Coregulation of Multiple Signaling Mechanisms in pp60v-Src-Induced Closure of Cx43 Gap Junction Channels. J Membr Biol 245, 495-506
22965738   Curated Info

8

Herrero-González S, et al. (2010) Connexin43 inhibits the oncogenic activity of c-Src in C6 glioma cells. Oncogene 29, 5712-23
20676131   Curated Info

9

Hu Y (2009) CST Curation Set: 8437; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Lin R, et al. (2006) v-Src tyrosine phosphorylation of connexin43: regulation of gap junction communication and effects on cell transformation. Cell Commun Adhes 13, 199-216
16916748   Curated Info

11

Warn-Cramer BJ, et al. (2003) Maintaining connexin43 gap junctional communication in v-Src cells does not alter growth properties associated with the transformed phenotype. Cell Commun Adhes 10, 299-303
14681032   Curated Info

12

Lin R, Warn-Cramer BJ, Kurata WE, Lau AF (2001) v-Src phosphorylation of connexin 43 on Tyr247 and Tyr265 disrupts gap junctional communication. J Cell Biol 154, 815-27
11514593   Curated Info

13

Giepmans BN, Hengeveld T, Postma FR, Moolenaar WH (2001) Interaction of c-Src with gap junction protein connexin-43. Role in the regulation of cell-cell communication. J Biol Chem 276, 8544-9
11124251   Curated Info