Ser725

Site Information
HtPKtDDsQEKtDDs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 475061

In vivo Characterization
Methods used to characterize site in vivo:	[32P] ATP in vitro ( 4 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 , 3 ) , phospho-antibody ( 4 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 1 ) , 293 (epithelial) ( 4 ) , HeLa (cervical) ( 4 ) , lung ( 3 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 2 ) , MEF (fibroblast) [ATM (mouse)] ( 4 ) , spleen ( 3 ) , testis ( 3 )

Downstream Regulation
Effects of modification on MCM3:	intracellular localization ( 4 )

References
1	Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36 27681418 Curated Info
2	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
3	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
4	Shi Y, et al. (2007) Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies. J Biol Chem 282, 9236-43 17244605 Curated Info