Tyr74
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Home > Phosphorylation Site Page: > Tyr74  -  p27Kip1 (human)

Site Information
HkPLEGKyEWQEVEk   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 475060

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 , 4 ) , mutation of modification site ( 1 , 2 , 7 ) , phospho-antibody ( 3 , 4 , 5 , 7 ) , western blotting ( 3 , 4 , 5 , 7 )
Disease tissue studied:
breast cancer ( 3 , 4 ) , lung cancer ( 5 ) , fibrosarcoma of soft tissue ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
Src (human) ( 1 , 7 )
Kinases, in vitro:
Src (human) ( 4 , 7 ) , Yes (human) ( 7 )
Treatments:
estradiol ( 7 ) , GGTI-2417 ( 3 ) , PP1 ( 7 )

Downstream Regulation
Effects of modification on p27Kip1:
molecular association, regulation ( 1 , 4 , 7 ) , protein degradation ( 2 , 7 )
Effects of modification on biological processes:
cell cycle regulation ( 1 , 7 )
Induce interaction with:
CCND1 (human) ( 4 ) , CDK4 (human) ( 4 )
Inhibit interaction with:
CCNE1 (human) ( 7 ) , CDK2 (human) ( 7 ) , CDK4 (human) ( 1 )

Disease / Diagnostics Relevance
Relevant diseases:
breast cancer ( 7 )

References 

1

Ou L, Waddell MB, Kriwacki RW (2012) Mechanism of cell cycle entry mediated by the intrinsically disordered protein p27(Kip1). ACS Chem Biol 7, 678-82
22276948   Curated Info

2

Schiappacassi M, et al. (2011) Role of T198 modification in the regulation of p27(Kip1) protein stability and function. PLoS One 6, e17673
21423803   Curated Info

3

Kazi A, et al. (2009) Blockade of protein geranylgeranylation inhibits Cdk2-dependent p27Kip1 phosphorylation on Thr187 and accumulates p27Kip1 in the nucleus: implications for breast cancer therapy. Mol Cell Biol 29, 2254-63
19204084   Curated Info

4

Larrea MD, et al. (2008) Phosphorylation of p27Kip1 regulates assembly and activation of cyclin D1-Cdk4. Mol Cell Biol 28, 6462-72
18710949   Curated Info

5

Lei K, Townsend DM, Tew KD (2008) Protein cysteine sulfinic acid reductase (sulfiredoxin) as a regulator of cell proliferation and drug response. Oncogene 27, 4877-87
18454177   Curated Info

6

James MK, Ray A, Leznova D, Blain SW (2008) Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity. Mol Cell Biol 28, 498-510
17908796   Curated Info

7

Chu I, et al. (2007) p27 phosphorylation by Src regulates inhibition of cyclin E-Cdk2. Cell 128, 281-94
17254967   Curated Info