Ser896
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.0.1
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser896  -  NMDAR1 (rat)

Site Information
ssFKRRRssKDtSTG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448255

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 19 ) , immunoassay ( 4 ) , mutation of modification site ( 5 , 8 , 19 ) , phospho-antibody ( 2 , 3 , 4 , 6 , 7 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 18 ) , phosphoamino acid analysis ( 19 ) , phosphopeptide mapping ( 7 , 19 ) , western blotting ( 2 , 3 , 4 , 6 , 11 , 12 , 14 , 16 )
Disease tissue studied:
colonic inflamation ( 3 ) , Parkinson's disease ( 7 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 4 , 6 , 12 , 15 , 18 ) , 'brain, striatum' ( 7 ) , 'brain, substantia nigra' ( 16 ) , 'neuron, cortical' ( 19 ) , 'neuron, cortical'-brain ( 10 ) , 'neuron, hippocampal, CA1 pyramidal'-brain ( 2 ) , 293 (epithelial) ( 5 , 8 , 18 , 19 ) , neuron-'brain, cerebral cortex' ( 11 ) , QT-6 (fibroblast) ( 18 ) , spinal cord ( 3 , 14 )

Upstream Regulation
Putative in vivo kinases:
PKCA (rat) ( 11 )
Kinases, in vitro:
PKCA (human) ( 17 ) , PKCA (rat) ( 18 )
Treatments:
6-OHDA ( 16 ) , AACOCF3 ( 9 ) , BDNF ( 3 ) , BEL ( 9 ) , bisindolylmaleimide ( 3 ) , capsaicin ( 14 ) , chelerythrine ( 14 ) , ciclosporin ( 10 ) , DHPG ( 11 ) , diabetes ( 4 ) , dopaminergic_lesion ( 7 ) , Go_6976 ( 11 ) , ischemia/reperfusion ( 15 ) , K252a ( 3 ) , levodopa ( 7 , 16 ) , LY294002 ( 3 ) , novel object recognition test ( 2 ) , PAF ( 6 ) , PCA4248 ( 6 ) , PD98059 ( 3 ) , PKC-theta_pseudosubstrate ( 11 ) , PKCalpha_peptide_inhibitor ( 11 ) , PKCbeta1_peptide_inhibitor ( 11 ) , PKCbeta2_peptide_inhibitor ( 11 ) , PKCgamma_peptide_inhibitor ( 11 ) , rottlerin ( 11 ) , status epilepticus ( 12 ) , stress ( 2 ) , tri-nitrobenzene_sulfonic_acid ( 3 ) , U73122 ( 3 ) , wortmannin ( 3 )

Downstream Regulation
Effects of modification on NMDAR1:
molecular association, regulation ( 17 )
Inhibit interaction with:
Calmodulin (rat) ( 17 )

Disease / Diagnostics Relevance
Relevant diseases:
Parkinson's disease ( 7 )

References 

1

Zhang Y, et al. (2022) Veratramine ameliorates pain symptoms in rats with diabetic peripheral neuropathy by inhibiting activation of the SIGMAR1-NMDAR pathway. Pharm Biol 60, 2145-2154
36373991   Curated Info

2

Calabrese F, et al. (2017) Chronic Mild Stress-Induced Alterations of Local Protein Synthesis: A Role for Cognitive Impairment. ACS Chem Neurosci 8, 817-825
28094500   Curated Info

3

Liu M, Kay JC, Shen S, Qiao LY (2015) Endogenous BDNF augments NMDA receptor phosphorylation in the spinal cord via PLCγ, PKC, and PI3K/Akt pathways during colitis. J Neuroinflammation 12, 151
26289587   Curated Info

4

Liao MH, et al. (2013) The Disturbance of Hippocampal CaMKII/PKA/PKC Phosphorylation in Early Experimental Diabetes Mellitus. CNS Neurosci Ther 19, 329-36
23490331   Curated Info

5

Xu M, Smothers CT, Woodward JJ (2011) Effects of ethanol on phosphorylation site mutants of recombinant N-methyl-D-aspartate receptors. Alcohol 45, 373-80
21163614   Curated Info

6

Moriguchi S, Shioda N, Yamamoto Y, Fukunaga K (2010) Platelet-activating factor-induced synaptic facilitation is associated with increased calcium/calmodulin-dependent protein kinase II, protein kinase C and extracellular signal-regulated kinase activities in the rat hippocampal CA1 region. Neuroscience 166, 1158-66
20074623   Curated Info

7

Kong M, Ba M, Song L, Liu Z (2009) Comparative effects of acute or chronic administration of levodopa to 6-OHDA-lesioned rats on the expression and phosphorylation of N-methyl-D-aspartate receptor NR1 subunits in the striatum. Neurochem Res 34, 1513-21
19283475   Curated Info

8

Jackson MF, et al. (2006) Protein kinase C enhances glycine-insensitive desensitization of NMDA receptors independently of previously identified protein kinase C sites. J Neurochem 96, 1509-18
16417568   Curated Info

9

Ménard C, Patenaude C, Massicotte G (2005) Phosphorylation of AMPA receptor subunits is differentially regulated by phospholipase A2 inhibitors. Neurosci Lett 389, 51-6
16099093   Curated Info

10

Choe ES, Shin EH, Wang JQ (2005) Inhibition of protein phosphatase 2B upregulates serine phosphorylation of N-methyl-D-aspartate receptor NR1 subunits in striatal neurons in vivo. Neurosci Lett 384, 38-43
15890444   Curated Info

11

Sánchez-Pérez AM, Felipo V (2005) Serines 890 and 896 of the NMDA receptor subunit NR1 are differentially phosphorylated by protein kinase C isoforms. Neurochem Int 47, 84-91
15936117   Curated Info

12

Niimura M, et al. (2005) Changes in phosphorylation of the NMDA receptor in the rat hippocampus induced by status epilepticus. J Neurochem 92, 1377-85
15748156   Curated Info

13

Ménard C, et al. (2005) AMPA receptor phosphorylation is selectively regulated by constitutive phospholipase A(2) and 5-lipoxygenase activities. Hippocampus 15, 370-80
15630695   Curated Info

14

Zou X, Lin Q, Willis WD (2004) Effect of protein kinase C blockade on phosphorylation of NR1 in dorsal horn and spinothalamic tract cells caused by intradermal capsaicin injection in rats. Brain Res 1020, 95-105
15312791   Curated Info

15

Cheung HH, Teves L, Wallace MC, Gurd JW (2001) Increased phosphorylation of the NR1 subunit of the NMDA receptor following cerebral ischemia. J Neurochem 78, 1179-82
11553692   Curated Info

16

Dunah AW, et al. (2000) Alterations in subunit expression, composition, and phosphorylation of striatal N-methyl-D-aspartate glutamate receptors in a rat 6-hydroxydopamine model of Parkinson's disease. Mol Pharmacol 57, 342-52
10648644   Curated Info

17

Hisatsune C, et al. (1997) Phosphorylation-dependent regulation of N-methyl-D-aspartate receptors by calmodulin. J Biol Chem 272, 20805-10
9252405   Curated Info

18

Tingley WG, et al. (1997) Characterization of protein kinase A and protein kinase C phosphorylation of the N-methyl-D-aspartate receptor NR1 subunit using phosphorylation site-specific antibodies. J Biol Chem 272, 5157-66
9030583   Curated Info

19

Tingley WG, Roche KW, Thompson AK, Huganir RL (1993) Regulation of NMDA receptor phosphorylation by alternative splicing of the C-terminal domain. Nature 364, 70-3
8316301   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2019 Cell Signaling Technology, Inc.