Lys254
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Home > Acetylation Site Page: > Lys254  -  GAPDH (human)

Site Information
CrLEkPAkyDDIkkV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 474649
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 3 ) , mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ) , modification-specific antibody ( 3 ) , mutation of modification site ( 3 ) , western blotting ( 3 )
Disease tissue studied:
colorectal cancer ( 10 , 11 ) , colorectal carcinoma ( 10 , 11 ) , leukemia ( 8 , 9 ) , chronic myelogenous leukemia ( 8 , 9 ) , lung cancer ( 2 , 3 ) , non-small cell lung cancer ( 3 , 6 ) , non-small cell lung adenocarcinoma ( 2 , 3 )
Relevant cell line - cell type - tissue:
A549 (pulmonary) ( 2 , 3 , 13 ) , Chang liver (cervical) ( 3 ) , HCT116 (intestinal) ( 10 , 11 ) , HEK293T (epithelial) ( 3 ) , HeLa (cervical) ( 1 , 4 ) , Jurkat (T lymphocyte) ( 5 , 13 , 14 , 15 ) , K562 (erythroid) ( 8 , 9 ) , liver ( 12 ) , MV4-11 (macrophage) ( 13 ) , NCI-H3255 (pulmonary) ( 6 )

Upstream Regulation
Treatments:
glucose ( 3 ) , nicotinamide ( 3 ) , siRNA ( 3 ) , trichostatin_A ( 3 )

Downstream Regulation
Effects of modification on GAPDH:
enzymatic activity, induced ( 3 )
Effects of modification on biological processes:
carcinogenesis, induced ( 3 ) , cell growth, induced ( 3 )

References 

1

Park SH, et al. (2016) SIRT2-Mediated Deacetylation and Tetramerization of Pyruvate Kinase Directs Glycolysis and Tumor Growth. Cancer Res 76, 3802-12
27197174   Curated Info

2

Wu Q, et al. (2015) Suberoylanilide hydroxamic acid treatment reveals crosstalks among proteome, ubiquitylome and acetylome in non-small cell lung cancer A549 cell line. Sci Rep 5, 9520
25825284   Curated Info

3

Li T, et al. (2014) Glyceraldehyde-3-phosphate Dehydrogenase Is Activated by Lysine 254 Acetylation in Response to Glucose Signal. J Biol Chem 289, 3775-85
24362262   Curated Info

4

Weinert BT, et al. (2013) Lysine succinylation is a frequently occurring modification in prokaryotes and eukaryotes and extensively overlaps with acetylation. Cell Rep 4, 842-51
23954790   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Rikova K (2012) CST Curation Set: 16031; Year: 2012; Biosample/Treatment: cell line, H3255/iressa; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK
Curated Info

7

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

8

Moritz A (2010) CST Curation Set: 9817; Year: 2010; Biosample/Treatment: cell line, K-562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK
Curated Info

9

Moritz A (2010) CST Curation Set: 9820; Year: 2010; Biosample/Treatment: cell line, K-562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK
Curated Info

10

Moritz A (2010) CST Curation Set: 9796; Year: 2010; Biosample/Treatment: cell line, HCT 116/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK
Curated Info

11

Moritz A (2010) CST Curation Set: 9797; Year: 2010; Biosample/Treatment: cell line, HCT 116/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK
Curated Info

12

Zhao S, et al. (2010) Regulation of cellular metabolism by protein lysine acetylation. Science 327, 1000-4
20167786   Curated Info

13

Choudhary C, et al. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science 325, 834-40
19608861   Curated Info

14

Possemato A (2006) CST Curation Set: 2193; Year: 2006; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK
Curated Info

15

Possemato A (2006) CST Curation Set: 2194; Year: 2006; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK
Curated Info

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