Ser62
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Home > Phosphorylation Site Page: > Ser62  -  Bcl-xL (human)

Site Information
PSWHLADsPAVNGAT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449099

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 7 ) , electrophoretic mobility shift ( 14 ) , immunoassay ( 7 ) , immunoprecipitation ( 3 , 7 , 11 ) , mass spectrometry ( 11 ) , mutation of modification site ( 3 , 7 , 11 , 13 , 14 ) , peptide sequencing ( 13 ) , phospho-antibody ( 1 , 4 , 5 , 7 , 8 , 9 , 10 , 12 , 14 ) , western blotting ( 1 , 3 , 4 , 5 , 7 , 8 , 9 , 11 , 12 , 13 )
Disease tissue studied:
cervical cancer ( 11 ) , cervical squamous cell carcinoma ( 11 ) , colorectal cancer ( 12 ) , colorectal carcinoma ( 12 ) , leukemia ( 4 ) , acute myelogenous leukemia ( 4 ) , liver cancer ( 1 ) , lung cancer ( 10 , 12 ) , non-small cell lung cancer ( 10 , 12 ) , non-small cell squamous cell lung carcinoma ( 12 ) , lymphoma ( 4 , 7 ) , Burkitt's lymphoma ( 7 ) , neuroblastoma ( 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
GSK3A (human) ( 7 ) , GSK3B (human) ( 7 ) , JNK1 (human) ( 7 ) , JNK2 (human) ( 7 ) , LKB1 (human) ( 12 ) , MAPKAPK2 (human) ( 7 ) , P38A (human) ( 7 ) , PLK1 (human) ( 7 ) , PPP2CA (human) ( 9 )
Putative in vivo kinases:
JNK1 (human) ( 14 ) , PINK1 (human) ( 5 )
Kinases, in vitro:
GSK3A (human) ( 7 ) , GSK3B (human) ( 7 ) , JNK1 (human) ( 7 ) , JNK2 (human) ( 7 ) , MAPKAPK2 (human) ( 7 ) , P38A (human) ( 7 ) , PLK1 (human) ( 7 )
Putative upstream phosphatases:
PGAM5 (human) ( 1 ) , PPP2CA (human) ( 9 )
Treatments:
2-deoxyglucose ( 10 ) , 2-methoxyestradiol ( 14 ) , BI2536 ( 7 ) , blebbistatin ( 8 ) , CCCP ( 5 ) , etoposide ( 7 ) , H2O2 ( 9 ) , kinetin ( 5 ) , MG132 ( 8 ) , navitoclax ( 4 ) , nocodazole ( 8 ) , NPD1 ( 9 ) , okadaic_acid ( 9 ) , oxindole-1 ( 4 ) , RO-3306 ( 4 ) , SB203580 ( 7 ) , SB216763 ( 7 ) , selenite ( 1 ) , seliciclib ( 4 ) , siRNA ( 5 , 12 ) , SP600125 ( 7 ) , taxol ( 14 ) , TNF ( 9 ) , vinblastine ( 1 , 11 )

Downstream Regulation
Effects of modification on Bcl-xL:
activity, induced ( 7 ) , intracellular localization ( 7 , 9 ) , molecular association, regulation ( 1 , 7 , 11 ) , protein conformation ( 2 )
Effects of modification on biological processes:
apoptosis, altered ( 12 , 13 ) , apoptosis, induced ( 1 , 11 , 14 ) , cell cycle regulation ( 3 , 7 , 13 ) , cell growth, inhibited ( 3 )
Induce interaction with:
CDK1 (human) ( 7 )
Inhibit interaction with:
BAK1 (human) ( 1 ) , BAX (human) ( 1 , 11 ) , CDK1 (human) ( 13 )

References 

1

Ma K, et al. (2019) Dynamic PGAM5 multimers dephosphorylate BCL-xL or FUNDC1 to regulate mitochondrial and cellular fate. Cell Death Differ
31367011   Curated Info

2

Priya P, Maity A, Ghosh Dastidar S (2017) The long unstructured region of Bcl-xl modulates its structural dynamics. Proteins 85, 1567-1579
28486788   Curated Info

3

Baruah PS, Beauchemin M, H├ębert J, Bertrand R (2016) Dynamic Bcl-xL (S49) and (S62) Phosphorylation/Dephosphorylation during Mitosis Prevents Chromosome Instability and Aneuploidy in Normal Human Diploid Fibroblasts. PLoS One 11, e0159091
27398719   Curated Info

4

Zhou W, et al. (2015) Inhibition of Bcl-xL overcomes polyploidy resistance and leads to apoptotic cell death in acute myeloid leukemia cells. Oncotarget 6, 21557-71
26188358   Curated Info

5

Hertz NT, et al. (2013) A Neo-Substrate that Amplifies Catalytic Activity of Parkinson's-Disease-Related Kinase PINK1. Cell 154, 737-47
23953109   Curated Info

6

Song X, Kim SY, Lee YJ (2013) Evidence for two modes of synergistic induction of apoptosis by mapatumumab and oxaliplatin in combination with hyperthermia in human colon cancer cells. PLoS One 8, e73654
24013390   Curated Info

7

Wang J, Beauchemin M, Bertrand R (2012) Phospho-Bcl-x(L)(Ser62) plays a key role at DNA damage-induced G(2) checkpoint. Cell Cycle 11, 2159-69
22617334   Curated Info

8

Wang J, Beauchemin M, Bertrand R (2011) Bcl-xL phosphorylation at Ser49 by polo kinase 3 during cell cycle progression and checkpoints. Cell Signal 23, 2030-8
21840391   Curated Info

9

Antony R, Lukiw WJ, Bazan NG (2010) Neuroprotectin D1 induces dephosphorylation of Bcl-xL in a PP2A-dependent manner during oxidative stress and promotes retinal pigment epithelial cell survival. J Biol Chem 285, 18301-8
20363734   Curated Info

10

Zhong D, et al. (2009) The Glycolytic Inhibitor 2-Deoxyglucose Activates Multiple Prosurvival Pathways through IGF1R. J Biol Chem 284, 23225-33
19574224   Curated Info

11

Upreti M, et al. (2008) Identification of the major phosphorylation site in Bcl-xL induced by microtubule inhibitors and analysis of its functional significance. J Biol Chem 283, 35517-25
18974096   Curated Info

12

Zhong D, et al. (2008) LKB1 is necessary for Akt-mediated phosphorylation of proapoptotic proteins. Cancer Res 68, 7270-7
18794113   Curated Info

13

Schmitt E, Beauchemin M, Bertrand R (2007) Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint. Oncogene 26, 5851-65
17369848   Curated Info

14

Basu A, Haldar S (2003) Identification of a novel Bcl-xL phosphorylation site regulating the sensitivity of taxol- or 2-methoxyestradiol-induced apoptosis. FEBS Lett 538, 41-7
12633850   Curated Info