Thr38
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Home > Phosphorylation Site Page: > Thr38  -  PPP1R14A (rat)

Site Information
QKRHARVtVkYDRRE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449098

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 8 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 8 ) , western blotting ( 1 , 2 , 3 , 5 , 6 )
Relevant cell line - cell type - tissue:
'muscle, smooth' ( 1 , 3 ) , 'muscle, smooth'-'heart, artery' ( 4 ) , cerebral artery ( 2 ) , VSMC-aorta ( 5 , 6 )

Upstream Regulation
Regulatory protein:
RHOA (human) ( 5 )
Kinases, in vitro:
PAK1 (human) ( 7 ) , PKN1 (rat) ( 8 )
Treatments:
angiotensin ( 6 ) , cGMP_analog ( 2 , 6 ) , endothelin ( 6 ) , exoenzyme_C3 ( 5 ) , GF109203X ( 5 , 6 ) , phorbol_ester ( 1 , 4 , 5 ) , thrombin ( 5 ) , U46619 ( 2 , 5 , 6 ) , Y27632 ( 3 , 5 , 6 )

Downstream Regulation
Effects of modification on PPP1R14A:
activity, inhibited ( 7 ) , phosphorylation ( 7 )

References 

1

Dougherty PJ, et al. (2014) PKC activation increases Ca²⁺ sensitivity of permeabilized lymphatic muscle via myosin light chain 20 phosphorylation-dependent and -independent mechanisms. Am J Physiol Heart Circ Physiol 306, H674-83
24414065   Curated Info

2

Neppl RL, et al. (2009) Thromboxane A2-induced bi-directional regulation of cerebral arterial tone. J Biol Chem 284, 6348-60
19095646   Curated Info

3

Patel CA, Rattan S (2006) Spontaneously tonic smooth muscle has characteristically higher levels of RhoA/ROK compared with the phasic smooth muscle. Am J Physiol Gastrointest Liver Physiol 291, G830-7
16763289   Curated Info

4

Wilson DP, et al. (2005) Thromboxane A2-induced contraction of rat caudal arterial smooth muscle involves activation of Ca2+ entry and Ca2+ sensitization: Rho-associated kinase-mediated phosphorylation of MYPT1 at Thr-855, but not Thr-697. Biochem J 389, 763-74
15823093   Curated Info

5

Pang H, et al. (2005) RhoA-Rho kinase pathway mediates thrombin- and U-46619-induced phosphorylation of a myosin phosphatase inhibitor, CPI-17, in vascular smooth muscle cells. Am J Physiol Cell Physiol 289, C352-60
15814590   Curated Info

6

Seko T, et al. (2003) Activation of RhoA and inhibition of myosin phosphatase as important components in hypertension in vascular smooth muscle. Circ Res 92, 411-8
12600888   Curated Info

7

Takizawa N, Koga Y, Ikebe M (2002) Phosphorylation of CPI17 and myosin binding subunit of type 1 protein phosphatase by p21-activated kinase. Biochem Biophys Res Commun 297, 773-8
12359219   Curated Info

8

Hamaguchi T, et al. (2000) Phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by protein kinase N. Biochem Biophys Res Commun 274, 825-30
10924361   Curated Info