Thr247
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr247  -  PRAS40 (rat)

Site Information
LPRPRLNtSDFQKLK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 470067

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 4 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 6 , 7 , 8 , 9 ) , phospho-antibody ( 1 , 4 , 5 , 10 ) , phosphopeptide mapping ( 4 ) , western blotting ( 1 , 4 , 5 , 10 )
Disease tissue studied:
adrenal cancer ( 6 , 7 , 8 ) , pheochromocytoma ( 6 , 7 , 8 ) , diabetes mellitus ( 10 ) , type 2 diabetes ( 10 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 1 , 10 ) , 3T3 (fibroblast) [INSR (human)] ( 10 ) , 3T3 (fibroblast) ( 5 ) , 3T3-L1 (fibroblast) ( 10 ) , adipose tissue ( 2 , 10 ) , brain ( 9 ) , H9c2 (myoblast) ( 10 ) , heart ( 2 , 10 ) , L6 (myoblast) ( 5 ) , liver ( 3 , 10 ) , muscle ( 2 ) , PC CL 3 (thyroid cell) ( 4 ) , PC-12 (chromaffin) ( 6 , 7 , 8 ) , stomach ( 2 )

Upstream Regulation
Kinases, in vitro:
Akt1 (rat) ( 4 )
Treatments:
6-MB-cAMP ( 4 ) , amino_acid_starvation ( 5 ) , calorie_restriction ( 1 ) , high-fat diet ( 5 ) , insulin ( 1 , 4 , 5 , 10 ) , low-fat diet ( 5 ) , LY294002 ( 10 ) , MK-2206 ( 1 ) , NKH_477 ( 4 ) , rapamycin ( 5 , 10 ) , U0126 ( 10 ) , wortmannin ( 5 , 10 )

Downstream Regulation
Effects of modification on PRAS40:
molecular association, regulation ( 4 )
Inhibit interaction with:
TORC1 (rat) ( 4 )

References 

1

Wang H, Arias EB, Cartee GD (2016) Calorie restriction leads to greater Akt2 activity and glucose uptake by insulin-stimulated skeletal muscle from old rats. Am J Physiol Regul Integr Comp Physiol 310, R449-58
26739650   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

4

Blancquaert S, et al. (2010) cAMP-dependent activation of mammalian target of rapamycin (mTOR) in thyroid cells. Implication in mitogenesis and activation of CDK4. Mol Endocrinol 24, 1453-68
20484410   Curated Info

5

Nascimento EB, et al. (2010) Phosphorylation of PRAS40 on Thr246 by PKB/AKT facilitates efficient phosphorylation of Ser183 by mTORC1. Cell Signal 22, 961-7
20138985   Curated Info

6

Zhou J (2009) CST Curation Set: 6823; Year: 2009; Biosample/Treatment: cell line, PC-12/forskolin; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

7

Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

8

Zhou J (2009) CST Curation Set: 6820; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

9

Guo A (2009) CST Curation Set: 5860; Year: 2009; Biosample/Treatment: tissue, brain/sham; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Nascimento EB, et al. (2006) Insulin-mediated phosphorylation of the proline-rich Akt substrate PRAS40 is impaired in insulin target tissues of high-fat diet-fed rats. Diabetes 55, 3221-8
17130464   Curated Info