Ser44
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Home > Phosphorylation Site Page: > Ser44  -  HEC1 (human)

Site Information
kPtFGkLsINkPtsE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 474539

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 , 4 , 5 , 9 ) , immunoprecipitation ( 2 , 6 ) , mass spectrometry ( 2 , 6 , 10 , 11 ) , microscopy-colocalization with upstream kinase ( 9 ) , mutation of modification site ( 2 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , western blotting ( 3 )
Disease tissue studied:
cervical cancer ( 6 ) , cervical adenocarcinoma ( 6 ) , colorectal cancer ( 4 ) , colorectal carcinoma ( 4 )
Relevant cell line - cell type - tissue:
HCT116 (intestinal) ( 4 ) , HeLa (cervical) ( 1 , 2 , 3 , 5 , 7 , 8 , 9 ) , HeLa K (cervical) ( 6 ) , HeLa S3 (cervical) [PLK1 (human), knockdown, Tet-inducible PLK1 siRNA] ( 11 ) , HeLa S3 (cervical) ( 11 ) , hTERT-RPE1 (epithelial) ( 9 ) , K562 (erythroid) ( 10 ) , PtK1 (epithelial) ( 6 )

Upstream Regulation
Regulatory protein:
AurB (human) ( 3 ) , Borealin (human) ( 1 ) , BUB1 (human) ( 9 ) , CASC5 (human) ( 9 ) , Haspin (human) ( 4 ) , HBXAP (human) ( 3 ) , HP1 alpha (human) ( 7 ) , INCENP (human) ( 5 )
Putative in vivo kinases:
AurB (human) ( 4 )
Kinases, in vitro:
AurA (human) ( 6 ) , AurB (human) ( 6 )
Treatments:
BAY-320 ( 4 ) , hyper-intrakinetochore_stretch ( 8 ) , MLN8054 ( 6 ) , monastrol ( 3 ) , nocodazole ( 2 , 4 ) , STLC ( 2 ) , TAME ( 2 ) , ZK-Thiazolidinone ( 11 ) , ZM447439 ( 3 , 4 , 6 , 7 )

Downstream Regulation
Effects of modification on biological processes:
cell cycle regulation ( 4 , 12 ) , cytoskeletal reorganization ( 2 )

References 

1

Sha L, et al. (2023) Non-canonical MLL1 activity regulates centromeric phase separation and genome stability. Nat Cell Biol 25, 1637-1649
37945831   Curated Info

2

Kucharski TJ, et al. (2022) Small changes in phospho-occupancy at the kinetochore-microtubule interface drive mitotic fidelity. J Cell Biol 221
35878017   Curated Info

3

Lee HS, et al. (2021) Spatiotemporal coordination of the RSF1-PLK1-Aurora B cascade establishes mitotic signaling platforms. Nat Commun 12, 5931
34635673   Curated Info

4

Hadders MA, et al. (2020) Untangling the contribution of Haspin and Bub1 to Aurora B function during mitosis. J Cell Biol 219
32027339   Curated Info

5

Yi Q, et al. (2018) Aurora B kinase activity-dependent and -independent functions of the chromosomal passenger complex in regulating sister chromatid cohesion. J Biol Chem
30523151   Curated Info

6

DeLuca KF, et al. (2018) Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore-microtubule dynamics. J Cell Biol 217, 163-177
29187526   Curated Info

7

Abe Y, et al. (2016) HP1-Assisted Aurora B Kinase Activity Prevents Chromosome Segregation Errors. Dev Cell 36, 487-97
26954544   Curated Info

8

Suzuki A, et al. (2014) The Architecture of CCAN Proteins Creates a Structural Integrity to Resist Spindle Forces and Achieve Proper Intrakinetochore Stretch. Dev Cell 30, 717-30
25268173   Curated Info

9

Caldas GV, DeLuca KF, DeLuca JG (2013) KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity. J Cell Biol 203, 957-69
24344188   Curated Info

10

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

11

Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457
20860994   Curated Info

12

DeLuca JG, et al. (2006) Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell 127, 969-82
17129782   Curated Info

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