Thr516
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Home > Phosphorylation Site Page: > Thr516  -  Tau (rat)

Site Information
ssPGsPGtPGsRSRt   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447933

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 9 ) , mass spectrometry ( 1 ) , mutation of modification site ( 1 , 10 ) , phospho-antibody ( 1 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , western blotting ( 1 , 5 , 6 , 7 , 8 , 9 , 10 )
Disease tissue studied:
Alzheimer's disease ( 7 , 10 , 11 ) , adrenal cancer ( 6 , 10 ) , pheochromocytoma ( 6 , 10 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 5 , 6 , 9 ) , 'neuron, cerebellar granule'-brain ( 11 ) , 'neuron, cortical'-brain ( 10 ) , 'neuron, hippocampal'-brain ( 10 ) , brain ( 7 , 8 , 12 ) , E.coli (bacterial) ( 1 ) , PC-12 (chromaffin) ( 6 , 10 )

Upstream Regulation
Regulatory protein:
APP (human) ( 10 )
Kinases, in vitro:
ERK2 (human) ( 1 )
Putative upstream phosphatases:
PPP2CA (rat) ( 9 )
Treatments:
anoxia ( 11 ) , DON ( 8 ) , fasting ( 8 ) , folic acid ( 9 ) , homocysteine ( 5 , 9 ) , hypoxia ( 3 ) , lithium ( 10 ) , okadaic_acid ( 7 , 8 , 12 ) , olomoucine ( 10 ) , PUGNAc ( 12 ) , seliciclib ( 10 ) , vitamin_B12 ( 9 ) , Zn(2+) ( 4 )

Downstream Regulation
Effects of modification on Tau:
molecular association, regulation ( 1 )

Disease / Diagnostics Relevance
Relevant diseases:
Alzheimer's disease ( 10 )

References 

1

Despres C, et al. (2017) Identification of the Tau phosphorylation pattern that drives its aggregation. Proc Natl Acad Sci U S A 114, 9080-9085
28784767   Curated Info

2

Chai GS, et al. (2014) Humanin attenuates Alzheimer-like cognitive deficits and pathological changes induced by amyloid β-peptide in rats. Neurosci Bull 30, 923-35
25391447   Curated Info

3

Zhang CE, et al. (2014) Hypoxia-induced tau phosphorylation and memory deficit in rats. Neurodegener Dis 14, 107-16
24993525   Curated Info

4

Xiong Y, et al. (2013) Zinc induces protein phosphatase 2A inactivation and tau hyperphosphorylation through Src dependent PP2A (tyrosine 307) phosphorylation. Neurobiol Aging 34, 745-56
22892311   Curated Info

5

Chai GS, et al. (2013) Betaine attenuates Alzheimer-like pathological changes and memory deficits induced by homocysteine. J Neurochem 124, 388-96
23157378   Curated Info

6

Lu Y, et al. (2011) Early growth response 1 (Egr-1) regulates phosphorylation of microtubule-associated protein tau in mammalian brain. J Biol Chem 286, 20569-81
21489990   Curated Info

7

Qian W, et al. (2010) PP2A regulates tau phosphorylation directly and also indirectly via activating GSK-3beta. J Alzheimers Dis 19, 1221-9
20308788   Curated Info

8

Liu F, et al. (2009) Reduced O-GlcNAcylation links lower brain glucose metabolism and tau pathology in Alzheimer's disease. Brain 132, 1820-32
19451179   Curated Info

9

Zhang CE, et al. (2008) Homocysteine induces tau phosphorylation by inactivating protein phosphatase 2A in rat hippocampus. Neurobiol Aging 29, 1654-65
17537547   Curated Info

10

Chang KA, et al. (2006) Phosphorylation of amyloid precursor protein (APP) at Thr668 regulates the nuclear translocation of the APP intracellular domain and induces neurodegeneration. Mol Cell Biol 26, 4327-38
16705182   Curated Info

11

Liu R, et al. (2005) Acute anoxia induces tau dephosphorylation in rat brain slices and its possible underlying mechanisms. J Neurochem 94, 1225-34
15992372   Curated Info

12

Liu F, et al. (2004) O-GlcNAcylation regulates phosphorylation of tau: a mechanism involved in Alzheimer's disease. Proc Natl Acad Sci U S A 101, 10804-9
15249677   Curated Info