Ser513
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.7
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser513  -  Tau (rat)

Site Information
SGyssPGsPGtPGsR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447928

In vivo Characterization
Methods used to characterize site in vivo:
amino acid analysis ( 25 ) , immunoprecipitation ( 16 ) , mass spectrometry ( 1 , 7 , 8 , 11 , 15 ) , mutation of modification site ( 1 , 17 ) , peptide sequencing ( 25 ) , phospho-antibody ( 1 , 4 , 9 , 10 , 12 , 13 , 14 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ) , phosphoamino acid analysis ( 25 ) , western blotting ( 1 , 4 , 6 , 9 , 10 , 12 , 13 , 14 , 16 , 17 , 21 , 22 )
Disease tissue studied:
Alzheimer's disease ( 13 , 17 , 19 ) , adrenal cancer ( 10 , 17 ) , pheochromocytoma ( 10 , 17 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 8 ) , 'brain, cerebellum' ( 8 ) , 'brain, cerebral cortex' ( 8 ) , 'brain, forebrain' ( 15 ) , 'brain, hippocampus' ( 9 , 10 , 12 , 16 , 21 ) , 'neuron, cerebellar granule'-brain ( 19 ) , 'neuron, cortical'-brain ( 17 , 24 ) , 'neuron, hippocampal' ( 6 ) , 'neuron, hippocampal'-brain ( 17 ) , adipose tissue ( 8 ) , blood ( 8 ) , brain ( 13 , 14 , 22 , 23 , 25 ) , E.coli (bacterial) ( 1 ) , heart ( 8 ) , intestine ( 8 ) , kidney ( 8 ) , liver ( 8 , 11 ) , lung ( 8 ) , muscle ( 8 ) , neuron-'brain, cerebral cortex' ( 4 ) , PC-12 (chromaffin) ( 7 , 10 , 17 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 7 ) , testis ( 8 )

Upstream Regulation
Regulatory protein:
APP (human) ( 17 )
Putative upstream phosphatases:
PPP2CA (rat) ( 16 )
Treatments:
anoxia ( 19 ) , beta-amyloid_25-35 ( 24 ) , calyculin_A ( 9 ) , Cdk2/5_inhibitor ( 22 ) , colchicine ( 24 ) , colforsin ( 22 ) , DON ( 14 ) , fasting ( 14 ) , folic acid ( 16 ) , ginsenoside ( 4 ) , glutamic acid ( 24 ) , H-CHO ( 18 ) , high-fat diet ( 12 ) , homocysteine ( 16 ) , hypoxia ( 5 ) , ischemia ( 12 ) , ischemic stroke ( 4 ) , isoproterenol ( 21 ) , lithium ( 17 , 22 ) , low_glucose ( 19 ) , LY294002 ( 4 ) , melatonin ( 21 ) , memantine ( 6 ) , okadaic_acid ( 6 , 13 , 14 , 23 , 24 ) , olomoucine ( 17 ) , PD98059 ( 22 ) , PUGNAc ( 23 ) , Rp-cAMPS ( 22 ) , SB203580 ( 22 ) , seliciclib ( 17 ) , streptozotocin ( 12 ) , tungstate ( 18 ) , vitamin_B12 ( 16 )

Downstream Regulation
Effects of modification on Tau:
molecular association, regulation ( 1 )

Disease / Diagnostics Relevance
Relevant diseases:
Alzheimer's disease ( 6 , 17 , 21 ) , diabetes mellitus ( 12 ) , type 2 diabetes ( 12 )

References 

1

Despres C, et al. (2017) Identification of the Tau phosphorylation pattern that drives its aggregation. Proc Natl Acad Sci U S A 114, 9080-9085
28784767   Curated Info

2

Chai GS, et al. (2014) Humanin attenuates Alzheimer-like cognitive deficits and pathological changes induced by amyloid β-peptide in rats. Neurosci Bull 30, 923-35
25391447   Curated Info

3

Kinoshita Y, et al. (2014) Role for NUP62 depletion and PYK2 redistribution in dendritic retraction resulting from chronic stress. Proc Natl Acad Sci U S A 111, 16130-5
25349423   Curated Info

4

Zhang X, et al. (2014) Ginsenoside Rd attenuates tau protein phosphorylation via the PI3K/AKT/GSK-3β pathway after transient forebrain ischemia. Neurochem Res 39, 1363-73
24792734   Curated Info

5

Zhang CE, et al. (2014) Hypoxia-induced tau phosphorylation and memory deficit in rats. Neurodegener Dis 14, 107-16
24993525   Curated Info

6

Zimmer ER, et al. (2012) Pretreatment with memantine prevents Alzheimer-like alterations induced by intrahippocampal okadaic Acid administration in rats. Curr Alzheimer Res 9, 1182-90
22515493   Curated Info

7

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

8

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

9

Yang X, et al. (2011) Melatonin ameliorates Alzheimer-like pathological changes and spatial memory retention impairment induced by calyculin A. J Psychopharmacol 25, 1118-25
20542922   Curated Info

10

Lu Y, et al. (2011) Early growth response 1 (Egr-1) regulates phosphorylation of microtubule-associated protein tau in mammalian brain. J Biol Chem 286, 20569-81
21489990   Curated Info

11

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

12

Zhang T, et al. (2010) Diabetes synergistically exacerbates poststroke dementia and tau abnormality in brain. Neurochem Int 56, 955-61
20398714   Curated Info

13

Qian W, et al. (2010) PP2A regulates tau phosphorylation directly and also indirectly via activating GSK-3beta. J Alzheimers Dis 19, 1221-9
20308788   Curated Info

14

Liu F, et al. (2009) Reduced O-GlcNAcylation links lower brain glucose metabolism and tau pathology in Alzheimer's disease. Brain 132, 1820-32
19451179   Curated Info

15

Edbauer D, et al. (2009) Identification and characterization of neuronal mitogen-activated protein kinase substrates using a specific phosphomotif antibody. Mol Cell Proteomics 8, 681-95
19054758   Curated Info

16

Zhang CE, et al. (2008) Homocysteine induces tau phosphorylation by inactivating protein phosphatase 2A in rat hippocampus. Neurobiol Aging 29, 1654-65
17537547   Curated Info

17

Chang KA, et al. (2006) Phosphorylation of amyloid precursor protein (APP) at Thr668 regulates the nuclear translocation of the APP intracellular domain and induces neurodegeneration. Mol Cell Biol 26, 4327-38
16705182   Curated Info

18

Gómez-Ramos A, et al. (2006) Sodium tungstate decreases the phosphorylation of tau through GSK3 inactivation. J Neurosci Res 83, 264-73
16397900   Curated Info

19

Liu R, et al. (2005) Acute anoxia induces tau dephosphorylation in rat brain slices and its possible underlying mechanisms. J Neurochem 94, 1225-34
15992372   Curated Info

20

Yamamoto H, et al. (2005) Phosphorylation of tau at serine 416 by Ca2+/calmodulin-dependent protein kinase II in neuronal soma in brain. J Neurochem 94, 1438-47
16000144   Curated Info

21

Wang XC, et al. (2005) Prevention of isoproterenol-induced tau hyperphosphorylation by melatonin in the rat. Sheng Li Xue Bao 57, 7-12
15719129   Curated Info

22

Liu SJ, et al. (2004) Tau becomes a more favorable substrate for GSK-3 when it is prephosphorylated by PKA in rat brain. J Biol Chem 279, 50078-88
15375165   Curated Info

23

Liu F, et al. (2004) O-GlcNAcylation regulates phosphorylation of tau: a mechanism involved in Alzheimer's disease. Proc Natl Acad Sci U S A 101, 10804-9
15249677   Curated Info

24

Davis DR, et al. (1995) The phosphorylation state of the microtubule-associated protein tau as affected by glutamate, colchicine and beta-amyloid in primary rat cortical neuronal cultures. Biochem J 309 ( Pt 3), 941-9
7639714   Curated Info

25

Watanabe A, et al. (1993) In vivo phosphorylation sites in fetal and adult rat tau. J Biol Chem 268, 25712-7
8245007   Curated Info