Ser2807
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Home > Phosphorylation Site Page: > Ser2807  -  RYR2 (mouse)

Site Information
YNRTRRIsQtsQVsI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448848

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 25 ) , mass spectrometry ( 4 , 10 , 20 , 23 , 26 , 27 , 30 ) , mutation of modification site ( 1 , 2 , 6 , 12 , 16 , 22 , 24 , 25 , 28 , 32 , 33 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 21 , 31 , 32 , 33 ) , western blotting ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 21 , 33 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 2 ) , 'heart, ventricle' ( 13 ) , 293 (epithelial) ( 8 , 16 , 28 , 31 , 33 ) , brain ( 1 ) , brain [RYR1 (mouse), genetic knockin] ( 22 ) , breast ( 1 ) , cardiac-heart ( 16 ) , heart ( 3 , 4 , 9 , 14 , 15 , 17 , 20 , 21 , 23 , 25 , 26 , 27 , 30 , 32 , 33 ) , heart [RYR2 (mouse), genetic knockin] ( 24 ) , HL-1 (myocyte) ( 11 ) , lung ( 23 ) , myocardium ( 6 ) , myocyte-heart ( 5 , 6 , 7 , 8 , 12 , 18 , 19 , 31 , 33 ) , neuron:postsynaptic density-'brain, hippocampus, CA1 region' ( 10 )

Upstream Regulation
Regulatory protein:
PKACA (human) ( 6 ) , PKACA (mouse) ( 9 ) , Spinophilin (mouse) ( 16 )
Putative in vivo kinases:
CAMK2A (human) ( 33 ) , PKACA (mouse) ( 32 , 33 )
Kinases, in vitro:
PKACA (human) ( 25 , 31 , 32 , 33 ) , PKG1 (human) ( 31 )
Treatments:
adriamycin ( 1 , 5 ) , autocamtide_inhibitory_peptide ( 17 ) , fructose-rich diet ( 8 ) , GLP-1 ( 11 ) , glutathione ( 13 ) , H2O2 ( 13 ) , heart failure ( 33 ) , inhibitor-1 ( 21 ) , ionizing_radiation ( 19 ) , isoproterenol ( 6 , 18 , 32 , 33 ) , mutation ( 7 ) , propranolol ( 2 ) , restraint stress ( 22 ) , S107 ( 1 , 22 ) , Sapogenins ( 6 ) , SD-208 ( 2 )

Downstream Regulation
Effects of modification on RYR2:
activity, induced ( 32 ) , molecular association, regulation ( 22 )
Effects of modification on biological processes:
exocytosis, altered ( 24 )
Inhibit interaction with:
FKBP1B (mouse) ( 22 )

Disease / Diagnostics Relevance
Relevant diseases:
cardiomyopathy ( 2 ) , Duchenne muscular dystrophy ( 24 ) , MI ( 2 )

References 

1

Liu Y, et al. (2023) Targeting ryanodine receptor type 2 to mitigate chemotherapy-induced neurocognitive impairments in mice. Sci Transl Med 15, eadf8977
37756377   Curated Info

2

Dridi H, et al. (2023) Heart failure-induced cognitive dysfunction is mediated by intracellular Ca leak through ryanodine receptor type 2. Nat Neurosci
37429912   Curated Info

3

Sun B, et al. (2021) Cardiac ryanodine receptor calcium release deficiency syndrome. Sci Transl Med 13
33536282   Curated Info

4

Campbell HM, et al. (2020) Loss of SPEG Inhibitory Phosphorylation of RyR2 Promotes Atrial Fibrillation. Circulation
32683896   Curated Info

5

Llach A, et al. (2019) Progression of excitation-contraction coupling defects in doxorubicin cardiotoxicity. J Mol Cell Cardiol 126, 129-139
30500377   Curated Info

6

Potenza DM, et al. (2018) Phosphorylation of the ryanodine receptor 2 at serine 2030 is required for a complete β-adrenergic response. J Gen Physiol
30541771   Curated Info

7

Okuda S, et al. (2018) CaMKII-mediated phosphorylation of RyR2 plays a crucial role in aberrant Carelease as an arrhythmogenic substrate in cardiac troponin T-related familial hypertrophic cardiomyopathy. Biochem Biophys Res Commun 496, 1250-1256
29402414   Curated Info

8

Federico M, et al. (2017) Calcium-calmodulin-dependent protein kinase mediates the intracellular signalling pathways of cardiac apoptosis in mice with impaired glucose tolerance. J Physiol 595, 4089-4108
28105734   Curated Info

9

Yang L, et al. (2016) Loss of β-adrenergic-stimulated phosphorylation of CaV1.2 channels on Ser1700 leads to heart failure. Proc Natl Acad Sci U S A 113, E7976-E7985
27864509   Curated Info

10

Li J, et al. (2016) Long-term potentiation modulates synaptic phosphorylation networks and reshapes the structure of the postsynaptic interactome. Sci Signal 9, rs8
27507650   Curated Info

11

Huang JH, et al. (2016) Glucagon-like peptide-1 regulates calcium homeostasis and electrophysiological activities of HL-1 cardiomyocytes. Peptides 78, 91-8
26930508   Curated Info

12

Wang Q, et al. (2015) Crosstalk between RyR2 oxidation and phosphorylation contributes to cardiac dysfunction in mice with Duchenne muscular dystrophy. J Mol Cell Cardiol 89, 177-84
26555638   Curated Info

13

Shirokova N, et al. (2014) Oxidative stress and ca(2+) release events in mouse cardiomyocytes. Biophys J 107, 2815-27
25517148   Curated Info

14

Respress JL, et al. (2014) Long-term simulated microgravity causes cardiac RyR2 phosphorylation and arrhythmias in mice. Int J Cardiol 176, 994-1000
25227892   Curated Info

15

Di Carlo MN, et al. (2014) CaMKII-dependent phosphorylation of cardiac ryanodine receptors regulates cell death in cardiac ischemia/reperfusion injury. J Mol Cell Cardiol 74, 274-83
24949568   Curated Info

16

Chiang DY, et al. (2014) Impaired local regulation of ryanodine receptor type 2 by protein phosphatase 1 promotes atrial fibrillation. Cardiovasc Res 103, 178-87
24812280   Curated Info

17

Chakraborty A, et al. (2014) Inhibition of CaMKII Does Not Attenuate Cardiac Hypertrophy in Mice with Dysfunctional Ryanodine Receptor. PLoS One 9, e104338
25093823   Curated Info

18

Besser J, et al. (2014) MiRNA-1/133a Clusters Regulate Adrenergic Control of Cardiac Repolarization. PLoS One 9, e113449
25415383   Curated Info

19

Sag CM, et al. (2013) Ionizing radiation regulates cardiac Ca handling via increased ROS and activated CaMKII. Basic Res Cardiol 108, 385
24068185   Curated Info

20

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

21

Pritchard TJ, et al. (2013) Active inhibitor-1 maintains protein hyper-phosphorylation in aging hearts and halts remodeling in failing hearts. PLoS One 8, e80717
24312496   Curated Info

22

Liu X, et al. (2012) Role of leaky neuronal ryanodine receptors in stress-induced cognitive dysfunction. Cell 150, 1055-67
22939628   Curated Info

23

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

24

Sarma S, et al. (2010) Genetic inhibition of PKA phosphorylation of RyR2 prevents dystrophic cardiomyopathy. Proc Natl Acad Sci U S A 107, 13165-70
20615971   Curated Info

25

Kushnir A, et al. (2010) Role of CaMKIIdelta phosphorylation of the cardiac ryanodine receptor in the force frequency relationship and heart failure. Proc Natl Acad Sci U S A 107, 10274-9
20479242   Curated Info

26

Zhou J (2009) CST Curation Set: 7385; Year: 2009; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: QXp[ST]
Curated Info

27

Zhou J (2009) CST Curation Set: 7386; Year: 2009; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: QXp[ST]
Curated Info

28

Xiao B, et al. (2007) Functional consequence of protein kinase A-dependent phosphorylation of the cardiac ryanodine receptor: sensitization of store overload-induced Ca2+ release. J Biol Chem 282, 30256-64
17693412   Curated Info

29

Meng X, et al. (2007) Three-dimensional localization of serine 2808, a phosphorylation site in cardiac ryanodine receptor. J Biol Chem 282, 25929-39
17606610   Curated Info

30

Guo A (2007) CST Curation Set: 2922; Year: 2007; Biosample/Treatment: tissue, heart/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

31

Xiao B, et al. (2006) Ser-2030, but not Ser-2808, is the major phosphorylation site in cardiac ryanodine receptors responding to protein kinase A activation upon beta-adrenergic stimulation in normal and failing hearts. Biochem J 396, 7-16
16483256   Curated Info

32

Wehrens XH, et al. (2006) Ryanodine receptor/calcium release channel PKA phosphorylation: a critical mediator of heart failure progression. Proc Natl Acad Sci U S A 103, 511-8
16407108   Curated Info

33

Xiao B, et al. (2005) Characterization of a novel PKA phosphorylation site, serine-2030, reveals no PKA hyperphosphorylation of the cardiac ryanodine receptor in canine heart failure. Circ Res 96, 847-55
15790957   Curated Info