Thr112
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Home > Phosphorylation Site Page: > Thr112  -  BIM (mouse)

Site Information
sCDKStQtPsPPCQA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449071

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 4 ) , flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mutation of modification site ( 1 , 3 , 4 ) , phospho-antibody ( 2 , 4 ) , western blotting ( 1 , 2 , 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 , 4 ) , B lymphocyte-spleen ( 3 ) , BEAS-2B (epithelial) ( 2 ) , epithelial ( 2 ) , epithelial-mammary gland ( 1 ) , HEK293T (epithelial) ( 1 ) , kidney ( 1 ) , MEF (fibroblast) ( 2 , 4 ) , T lymphocyte-spleen ( 3 )

Upstream Regulation
Putative in vivo kinases:
ERK1 (mouse) ( 4 ) , ERK2 (mouse) ( 4 ) , JNK1 (mouse) ( 2 , 4 )
Treatments:
gliotoxin ( 2 ) , serum ( 4 ) , siRNA ( 2 ) , SP600125 ( 2 ) , U0126 ( 4 ) , UV ( 4 )

Downstream Regulation
Effects of modification on BIM:
molecular association, regulation ( 2 , 4 ) , protein stabilization ( 2 )
Effects of modification on biological processes:
apoptosis, altered ( 3 ) , apoptosis, induced ( 2 , 4 )
Induce interaction with:
Bcl-2 (human) ( 2 ) , Bcl-2 (mouse) ( 4 ) , Bcl-xL (human) ( 2 )

References 

1

Girnius N, Davis RJ (2017) JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins. Cell Rep 21, 1910-1921
29141222   Curated Info

2

Geissler A, et al. (2013) Apoptosis induced by the fungal pathogen gliotoxin requires a triple phosphorylation of Bim by JNK. Cell Death Differ 20, 1317-29
23832115   Curated Info

3

Hübner A, et al. (2010) Functional cooperation of the proapoptotic Bcl2 family proteins Bmf and Bim in vivo. Mol Cell Biol 30, 98-105
19841067   Curated Info

4

Hübner A, Barrett T, Flavell RA, Davis RJ (2008) Multisite phosphorylation regulates Bim stability and apoptotic activity. Mol Cell 30, 415-25
18498746   Curated Info