Tyr114
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Home > Phosphorylation Site Page: > Tyr114  -  PHB (human)

Site Information
FtsIGEDyDERVLPs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 6374001

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 , 5 ) , mass spectrometry ( 1 ) , mutation of modification site ( 2 , 3 , 4 , 5 ) , phospho-antibody ( 4 ) , western blotting ( 4 , 5 )
Disease tissue studied:
lung cancer ( 1 , 2 ) , non-small cell lung adenocarcinoma ( 1 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 3 ) , C2C12 (myoblast) ( 4 ) , CL1-0 (pulmonary) ( 2 ) , HeLa (cervical) ( 2 ) , PC9 (pulmonary) ( 1 ) , RINm5F ( 5 )

Upstream Regulation
Kinases, in vitro:
INSR (human) ( 4 )
Treatments:
insulin ( 4 , 5 )

Downstream Regulation
Effects of modification on PHB:
molecular association, regulation ( 4 ) , O-GlcNAc glycosylation ( 5 ) , phosphorylation ( 3 , 4 )
Effects of modification on biological processes:
cell differentiation, induced ( 3 ) , transcription, altered ( 3 )
Induce interaction with:
SHP-1 (human) ( 4 )

References 

1

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

2

Chiu CF, et al. (2013) Raf activation by Ras and promotion of cellular metastasis require phosphorylation of prohibitin in the raft domain of the plasma membrane. Oncogene 32, 777-87
22410782   Curated Info

3

Ande SR, Xu Z, Gu Y, Mishra S (2012) Prohibitin has an important role in adipocyte differentiation. Int J Obes (Lond) 36, 1236-44
22124450   Curated Info

4

Ande SR, Gu Y, Nyomba BL, Mishra S (2009) Insulin induced phosphorylation of prohibitin at tyrosine114 recruits Shp1. Biochim Biophys Acta 1793, 1372-8
19497338   Curated Info

5

Ande SR, Moulik S, Mishra S (2009) Interaction between O-GlcNAc Modification and Tyrosine Phosphorylation of Prohibitin: Implication for a Novel Binary Switch. PLoS One 4, e4586
19238206   Curated Info