Ser173
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Home > Phosphorylation Site Page: > Ser173  -  IRF3 (human)

Site Information
PCPQPLRsPsLDNPt   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 6370303

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 ) , immunoprecipitation ( 1 , 5 , 8 ) , mass spectrometry ( 1 , 2 , 3 , 7 , 8 ) , mass spectrometry (in vitro) ( 7 ) , mutation of modification site ( 1 , 4 , 5 , 8 ) , phospho-antibody ( 1 ) , western blotting ( 1 , 5 , 8 )
Disease tissue studied:
breast cancer ( 3 ) , breast ductal carcinoma ( 3 ) , ovarian cancer ( 3 ) , melanoma skin cancer ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 4 , 7 ) , breast ( 3 ) , HEK293T (epithelial) ( 1 , 5 , 8 ) , macrophage-bone marrow ( 1 ) , MEF (fibroblast) ( 1 ) , ovary ( 3 ) , WM239A (melanocyte) ( 2 )

Upstream Regulation
Regulatory protein:
TAK1 (human) ( 8 )
Putative in vivo kinases:
Cot (human) ( 5 ) , GSK3B (human) ( 4 ) , JNK1 (human) ( 8 ) , MEKK2 (human) ( 1 )
Kinases, in vitro:
GSK3A (human) ( 4 ) , GSK3B (human) ( 4 ) , JNK1 (human) ( 8 ) , TBK1 (human) ( 7 )
Treatments:
lithium ( 4 ) , selumetinib ( 2 ) , SP600125 ( 8 ) , vemurafenib ( 2 )

Downstream Regulation
Effects of modification on IRF3:
molecular association, regulation ( 5 ) , phosphorylation ( 8 )
Effects of modification on biological processes:
transcription, altered ( 8 ) , transcription, inhibited ( 4 , 5 )
Induce interaction with:
IRF7 (human) ( 5 )

References 

1

Gao L, et al. (2018) Tumor-derived exosomes antagonize innate antiviral immunity. Nat Immunol
29358709   Curated Info

2

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Wang JT, et al. (2014) Glycogen synthase kinase 3 negatively regulates IFN regulatory factor 3 transactivation through phosphorylation at its linker region. Innate Immun 20, 78-87
23685991   Curated Info

5

Schmid S, Sachs D, tenOever BR (2014) Mitogen-activated protein kinase-mediated licensing of interferon regulatory factor 3/7 reinforces the cell response to virus. J Biol Chem 289, 299-311
24275658   Curated Info

6

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

7

Bergstroem B, et al. (2010) Identification of a novel in vivo virus-targeted phosphorylation site in interferon regulatory factor-3 (IRF3). J Biol Chem 285, 24904-14
20511230   Curated Info

8

Zhang B, et al. (2009) The TAK1-JNK cascade is required for IRF3 function in the innate immune response. Cell Res 19, 412-28
19153595   Curated Info