Thr381
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Home > Phosphorylation Site Page: > Thr381  -  YTHDF2 (human)

Site Information
SQAGSGstPsEPHPV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 6223825
Associated spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 4 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
brain cancer ( 1 ) , glioblastoma ( 1 ) , glioma ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
EGFR (human) ( 1 )
Putative in vivo kinases:
ERK1 (human) ( 1 ) , ERK2 (human) ( 1 )
Kinases, in vitro:
ERK1 (human) ( 1 )
Treatments:
CGP57380 ( 1 ) , gefitinib ( 1 ) , SCH772984 ( 1 )

Downstream Regulation
Effects of modification on YTHDF2:
protein stabilization ( 1 )
Effects of modification on biological processes:
apoptosis, inhibited ( 1 ) , carcinogenesis, induced ( 1 ) , cell growth, induced ( 1 ) , RNA stability, inhibited ( 1 )

References 

1

Fang R, et al. (2021) EGFR/SRC/ERK-stabilized YTHDF2 promotes cholesterol dysregulation and invasive growth of glioblastoma. Nat Commun 12, 177
33420027   Curated Info

2

Guo A (2011) CST Curation Set: 11453; Year: 2011; Biosample/Treatment: cell line, HeLa/nocodazole &'||' pervanadate; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb Cat#: 5243, PTMScan(R) Phospho-PLK Binding Motif (ST*P) Immunoaffinity Beads Cat#: 5756
Curated Info

3

Possemato A (2010) CST Curation Set: 9437; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb Cat#: 5243, PTMScan(R) Phospho-PLK Binding Motif (ST*P) Immunoaffinity Beads Cat#: 5756
Curated Info

4

Possemato A (2008) CST Curation Set: 5274; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info