Ser398
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Home > Phosphorylation Site Page: > Ser398  -  IRF3 (human)

Site Information
VDLHIsNsHPLsLts   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449029

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 8 ) , immunoprecipitation ( 4 ) , mass spectrometry (in vitro) ( 3 ) , mutation of modification site ( 1 , 2 , 4 , 5 , 8 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
IKKB (human) ( 4 ) , TBK1 (human) ( 4 )
Kinases, in vitro:
IKKE (human) ( 3 , 6 ) , TBK1 (human) ( 6 )
Treatments:
virus infection ( 5 , 8 )

Downstream Regulation
Effects of modification on IRF3:
activity, induced ( 5 ) , intracellular localization ( 8 ) , molecular association, regulation ( 7 , 8 )
Effects of modification on biological processes:
transcription, altered ( 4 ) , transcription, induced ( 1 , 2 )
Induce interaction with:
CBP (human) ( 8 ) , IRF3 (human) ( 7 )

References 

1

Liu B, et al. (2017) The ubiquitin E3 ligase TRIM31 promotes aggregation and activation of the signaling adaptor MAVS through Lys63-linked polyubiquitination. Nat Immunol 18, 214-224
27992402   Curated Info

2

Zhang L, et al. (2014) Ubiquitin-Specific Protease 2b Negatively Regulates IFN-β Production and Antiviral Activity by Targeting TANK-Binding Kinase 1. J Immunol 193, 2230-7
25070846   Curated Info

3

Takahasi K, et al. (2010) Ser386 phosphorylation of transcription factor IRF-3 induces dimerization and association with CBP/p300 without overall conformational change. Genes Cells 15, 901-10
20604809   Curated Info

4

Zhang B, et al. (2009) The TAK1-JNK cascade is required for IRF3 function in the innate immune response. Cell Res 19, 412-28
19153595   Curated Info

5

Clément JF, et al. (2008) Phosphorylation of IRF-3 on Ser 339 generates a hyperactive form of IRF-3 through regulation of dimerization and CBP association. J Virol 82, 3984-96
18272581   Curated Info

6

Paz S, et al. (2006) Induction of IRF-3 and IRF-7 phosphorylation following activation of the RIG-I pathway. Cell Mol Biol (Noisy-le-grand) 52, 17-28
16914100   Curated Info

7

Mori M, et al. (2004) Identification of Ser-386 of interferon regulatory factor 3 as critical target for inducible phosphorylation that determines activation. J Biol Chem 279, 9698-702
14703513   Curated Info

8

Lin R, Heylbroeck C, Pitha PM, Hiscott J (1998) Virus-dependent phosphorylation of the IRF-3 transcription factor regulates nuclear translocation, transactivation potential, and proteasome-mediated degradation. Mol Cell Biol 18, 2986-96
9566918   Curated Info