Lys477

Site Information
PERREARkyASGLLk SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 610815806

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 2 ) , microscopy-colocalization with upstream kinase ( 1 ) , modification-specific antibody ( 1 ) , mutation of modification site ( 1 ) , western blotting ( 1 )
Disease tissue studied:	breast cancer ( 1 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 1 ) , HEp-2 (squamous) ( 2 ) , Jurkat (T lymphocyte) ( 2 ) , MDA-MB-231 (breast cell) ( 1 )

Downstream Regulation
Effects of modification on DVL2:	intracellular localization ( 1 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:	cell motility, induced ( 1 ) , signaling pathway regulation ( 1 )
Induce interaction with:	Cofilin-1 (human) ( 1 ) , VANGL1 (human) ( 1 )

References
1	Nielsen CP, et al. (2019) USP9X Deubiquitylates DVL2 to Regulate WNT Pathway Specification. Cell Rep 28, 1074-1089.e5 31340145 Curated Info
2	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
3	Lumpkin RJ, et al. (2017) Site-specific identification and quantitation of endogenous SUMO modifications under native conditions. Nat Commun 8, 1171 29079793 Curated Info