Tyr4
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Home > Phosphorylation Site Page: > Tyr4  -  CDK1 (human)

Site Information
____MEDytkIEkIG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 473276

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 4 , 5 , 6 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 1 ) , peptide sequencing ( 1 ) , western blotting ( 1 )
Disease tissue studied:
esophageal cancer ( 3 ) , esophageal carcinoma ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
PKR (human) ( 1 )
Kinases, in vitro:
PKR (human) ( 1 )

Downstream Regulation
Effects of modification on CDK1:
protein degradation ( 1 ) , ubiquitination ( 1 )
Effects of modification on biological processes:
cell cycle regulation ( 1 )

References 

1

Yoon CH, Miah MA, Kim KP, Bae YS (2010) New Cdc2 Tyr 4 phosphorylation by dsRNA-activated protein kinase triggers Cdc2 polyubiquitination and G2 arrest under genotoxic stresses. EMBO Rep 11, 393-9
20395957   Curated Info

2

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

3

Yu J (2006) CST Curation Set: 1365; Year: 2006; Biosample/Treatment: cell line, Kyse70/serum starved; Disease: esophageal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Farnsworth C (2006) CST Curation Set: 1078; Year: 2006; Biosample/Treatment: cell line, DMS153/serum starved &'||' 10% serum; Disease: small-cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Farnsworth C (2006) CST Curation Set: 1079; Year: 2006; Biosample/Treatment: cell line, DMS153/serum starved &'||' conditined media; Disease: small-cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Farnsworth C (2006) CST Curation Set: 1077; Year: 2006; Biosample/Treatment: cell line, DMS153/serum starved; Disease: small-cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info