Ser149

Site Information
GRSGRGksFtLtItV SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 6097701

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 2 ) , mass spectrometry ( 1 , 2 ) , western blotting ( 2 )
Disease tissue studied:	hepatocellular carcinoma, surrounding tissue ( 1 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 2 ) , COS (fibroblast) ( 2 ) , hepatic-'liver, para-hepatocellular carcinoma tissue' ( 1 )

Upstream Regulation
Putative in vivo kinases:	Pim1 (human) ( 2 )

Downstream Regulation
Effects of modification on AML2:	intracellular localization ( 2 ) , protein stabilization ( 2 )
Effects of modification on biological processes:	cell cycle regulation ( 2 ) , transcription, altered ( 2 )

References
1	Gu T (2009) CST Curation Set: 6581; Year: 2009; Biosample/Treatment: liver, para-hepatocellular carcinoma tissue, hepatic/untreated; Disease: liver cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
2	Kim HR, Oh BC, Choi JK, Bae SC (2008) Pim-1 kinase phosphorylates and stabilizes RUNX3 and alters its subcellular localization. J Cell Biochem 105, 1048-58 18767071 Curated Info

References

Gu T (2009) CST Curation Set: 6581; Year: 2009; Biosample/Treatment: liver, para-hepatocellular carcinoma tissue, hepatic/untreated; Disease: liver cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

Kim HR, Oh BC, Choi JK, Bae SC (2008) Pim-1 kinase phosphorylates and stabilizes RUNX3 and alters its subcellular localization. J Cell Biochem 105, 1048-58
18767071 Curated Info