Thr180
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Home > Phosphorylation Site Page: > Thr180  -  MST2 (human)

Site Information
DtMAkRNtVIGTPFW   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448899

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 7 , 8 , 9 , 10 , 11 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 )
Disease tissue studied:
bone cancer ( 2 ) , brain cancer ( 4 ) , Schwannoma ( 4 ) , breast cancer ( 1 , 5 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 ) , lung cancer ( 1 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 ) , ovarian cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
Akt1 (human) ( 6 ) , Ezrin (human) ( 3 ) , FRMD6 (human) ( 3 ) , LKB1 (human) ( 1 ) , Merlin (human) ( 3 , 4 )
Putative in vivo kinases:
MST2 (human) ( 2 , 6 )
Treatments:
adriamycin ( 6 ) , API-2 ( 6 ) , doxycycline ( 1 ) , IGF-1 ( 6 ) , LY294002 ( 6 ) , staurosporine ( 4 , 6 ) , Z-VAD-FMK ( 4 , 6 )

Downstream Regulation
Effects of modification on MST2:
enzymatic activity, induced ( 2 , 5 , 6 ) , molecular association, regulation ( 2 ) , phosphorylation ( 2 ) , protein processing ( 6 )
Effects of modification on biological processes:
apoptosis, altered ( 6 ) , signaling pathway regulation ( 1 , 2 , 5 )
Induce interaction with:
LATS1 (human) ( 2 )

References 

1

Mohseni M, et al. (2014) A genetic screen identifies an LKB1-MARK signalling axis controlling the Hippo-YAP pathway. Nat Cell Biol 16, 108-17
24362629   Curated Info

2

Yee KS, et al. (2012) A RASSF1A Polymorphism Restricts p53/p73 Activation and Associates with Poor Survival and Accelerated Age of Onset of Soft Tissue Sarcoma. Cancer Res 72, 2206-17
22389451   Curated Info

3

Angus L, et al. (2012) Willin/FRMD6 expression activates the Hippo signaling pathway kinases in mammals and antagonizes oncogenic YAP. Oncogene 31, 238-50
21666719   Curated Info

4

Kilili GK, Kyriakis JM (2010) Mammalian Ste20-like kinase (Mst2) indirectly supports Raf-1/ERK pathway activity via maintenance of protein phosphatase-2A catalytic subunit levels and consequent suppression of inhibitory Raf-1 phosphorylation. J Biol Chem 285, 15076-87
20212043   Curated Info

5

Romano D, et al. (2010) Proapoptotic kinase MST2 coordinates signaling crosstalk between RASSF1A, Raf-1, and Akt. Cancer Res 70, 1195-203
20086174   Curated Info

6

Kim D, et al. (2010) Regulation of proapoptotic mammalian ste20-like kinase MST2 by the IGF1-Akt pathway. PLoS One 5, e9616
20231902   Curated Info

7

Zhou J (2009) CST Curation Set: 7590; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

8

Zhou J (2009) CST Curation Set: 7593; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

9

Zhou J (2009) CST Curation Set: 7592; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

10

Zhou J (2009) CST Curation Set: 7591; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

11

Zhou J (2009) CST Curation Set: 7589; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

12

Praskova M, Xia F, Avruch J (2008) MOBKL1A/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation. Curr Biol 18, 311-21
18328708   Curated Info