Ser342

Site Information
DDGkLDLsVVENGGL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 5938010

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , mutation of modification site ( 6 , 7 ) , phospho-antibody ( 7 ) , western blotting ( 6 , 7 )
Disease tissue studied:	cervical cancer ( 5 ) , cervical adenocarcinoma ( 5 ) , lung cancer ( 7 ) , non-small cell lung cancer ( 7 )
Relevant cell line - cell type - tissue:	HEK293T (epithelial) ( 6 ) , HeLa (cervical) ( 1 , 4 ) , HeLa S3 (cervical) ( 5 ) , Jurkat (T lymphocyte) ( 2 ) , K562 (erythroid) ( 3 ) , NCI-H1299 (pulmonary) ( 7 )

Downstream Regulation
Effects of modification on biological processes:	cytoskeletal reorganization ( 6 )

References
1	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
2	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
3	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
4	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
5	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
6	López-Sánchez I, Sanz-García M, Lazo PA (2009) Plk3 interacts with and specifically phosphorylates VRK1 in Ser342, a downstream target in a pathway that induces Golgi fragmentation. Mol Cell Biol 29, 1189-201 19103756 Curated Info
7	Valbuena A, et al. (2007) Identification of a dominant epitope in human vaccinia-related kinase 1 (VRK1) and detection of different intracellular subpopulations. Arch Biochem Biophys 465, 219-26 17617371 Curated Info