Ser552
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Home > Phosphorylation Site Page: > Ser552  -  CTNNB1 (human)

Site Information
QDtQRRtsMGGtQQQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 472273

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 53 ) , immunoprecipitation ( 3 , 11 , 52 ) , mass spectrometry ( 1 , 2 , 7 , 8 , 10 , 12 , 13 , 14 , 15 , 17 , 18 , 19 , 20 , 21 , 22 , 24 , 25 , 26 , 29 , 30 , 31 , 33 , 34 , 35 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ) , mutation of modification site ( 5 , 11 , 52 , 53 ) , phospho-antibody ( 3 , 5 , 6 , 11 , 28 , 32 , 36 , 37 , 40 , 52 ) , western blotting ( 3 , 5 , 6 , 11 , 28 , 36 , 37 , 40 , 52 )
Disease tissue studied:
bone cancer ( 39 ) , osteosarcoma ( 39 ) , breast cancer ( 5 , 7 , 14 , 15 , 24 ) , breast ductal carcinoma ( 14 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 , 14 ) , colorectal cancer ( 37 , 40 ) , colorectal carcinoma ( 37 , 40 ) , kidney cancer ( 36 ) , hepatocellular carcinoma, surrounding tissue ( 38 ) , lung cancer ( 12 , 20 , 24 ) , non-small cell lung cancer ( 24 ) , non-small cell lung adenocarcinoma ( 12 , 20 ) , ovarian cancer ( 6 , 14 ) , pancreatic ductal adenocarcinoma ( 19 ) , melanoma skin cancer ( 10 )
Relevant cell line - cell type - tissue:
'pancreatic, ductal'-pancreas ( 19 ) , 293 (epithelial) ( 13 , 42 , 53 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 34 ) , 293 (epithelial) [AT1 (human), transfection] ( 33 ) , 293T (epithelial) ( 52 ) , 786-O (renal) ( 8 ) , 786-O (renal) [VHL (human), transfection] ( 8 ) , A431 (epithelial) ( 52 ) , A498 (renal) ( 35 ) , A498 (renal) [SFRP2 (human), transfection] ( 36 ) , A549 (pulmonary) ( 21 ) , breast ( 2 , 14 ) , BT-20 (breast cell) ( 24 ) , BT-549 (breast cell) ( 24 ) , Calu 6 (pulmonary) ( 24 ) , colon ( 32 ) , COS (fibroblast) ( 53 ) , endothelial-aorta ( 25 ) , Flp-In T-Rex-293 (epithelial) ( 26 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 26 ) , H2009 (pulmonary) ( 24 ) , H2077 (pulmonary) ( 24 ) , H2887 (pulmonary) ( 24 ) , H322 (pulmonary) ( 24 ) , H322M (pulmonary) ( 24 ) , HCC1359 (pulmonary) ( 24 ) , HCC1937 (breast cell) ( 24 ) , HCC2279 (pulmonary) ( 24 ) , HCC366 (pulmonary) ( 24 ) , HCC4006 (pulmonary) ( 24 ) , HCC78 (pulmonary) ( 24 ) , HCC827 (pulmonary) ( 24 ) , HeLa (cervical) ( 1 , 3 , 43 , 44 , 51 ) , hepatocyte-liver ( 38 ) , HMLER ('stem, breast cancer') ( 15 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 15 ) , HOP62 (pulmonary) ( 24 ) , HUES-7 ('stem, embryonic') ( 41 ) , HUES-9 ('stem, embryonic') ( 30 ) , Jurkat (T lymphocyte) ( 22 , 29 , 31 , 45 ) , LCLC-103H (pulmonary) ( 24 ) , liver ( 18 ) , LOU-NH91 (squamous) ( 24 ) , lung ( 20 ) , MCF-7 (breast cell) ( 5 , 7 , 24 ) , MDA-MB-231 (breast cell) ( 24 ) , MDA-MB-468 (breast cell) ( 24 ) , MKN-45 (gastric) ( 48 , 49 ) , NCI-H1395 (pulmonary) ( 24 ) , NCI-H1568 (pulmonary) ( 24 ) , NCI-H157 (pulmonary) ( 24 ) , NCI-H1648 (pulmonary) ( 24 ) , NCI-H1666 (pulmonary) ( 24 ) , NCI-H2030 (pulmonary) ( 24 ) , NCI-H2172 (pulmonary) ( 24 ) , NCI-H3255 (pulmonary) ( 46 , 47 ) , NCI-H460 (pulmonary) ( 24 ) , NCI-H520 (squamous) ( 24 ) , NCI-H647 (pulmonary) ( 24 ) , NCMiCaSR (endothelial) [CaR (human), genetic knockin] ( 28 ) , ovary ( 14 ) , PC9 (pulmonary) ( 12 , 24 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 17 ) , SKOV-3 (ovarian) ( 6 ) , SUM159 (breast cell) ( 5 ) , SW480 (intestinal) ( 40 ) , T84 (intestinal) ( 37 ) , TERT20 ('stem, mesenchymal') ( 50 ) , U2OS (bone cell) ( 39 ) , WM239A (epidermal) ( 10 )

Upstream Regulation
Regulatory protein:
ARFGEF1 (human) ( 3 ) , ARFGEF2 (human) ( 3 ) , CaR (human) ( 28 ) , Merlin (human) ( 5 ) , SFRP2 (human) ( 36 )
Putative in vivo kinases:
Akt1 (human) ( 52 ) , Akt2 (human) ( 52 )
Kinases, in vitro:
Akt1 (human) ( 52 ) , Akt2 (human) ( 52 ) , CAMK2A (human) ( 16 ) , PKACA (human) ( 53 )
Treatments:
angiotensin_2 ( 34 ) , Ca(2+) ( 28 ) , colforsin ( 53 ) , EGF ( 28 , 52 ) , GSK690693 ( 28 ) , IFN-gamma ( 37 ) , KT5720 ( 28 ) , LY294002 ( 6 ) , microparticles ( 6 ) , NSC23766 ( 6 ) , tecalcet ( 28 ) , TNF ( 37 )

Downstream Regulation
Effects of modification on CTNNB1:
intracellular localization ( 28 , 40 , 52 )
Effects of modification on biological processes:
apoptosis, induced ( 16 ) , cell motility, altered ( 52 ) , transcription, induced ( 52 ) , transcription, inhibited ( 16 )
Induce interaction with:
14-3-3 zeta (human) ( 52 )

Disease / Diagnostics Relevance
Relevant diseases:
colonic inflamation ( 32 )

References 

1

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Li CC, et al. (2016) Enhancement of β-catenin activity by BIG1 plus BIG2 via Arf activation and cAMP signals. Proc Natl Acad Sci U S A 113, 5946-51
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4

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

5

Morrow KA, et al. (2016) Loss of tumor suppressor Merlin results in aberrant activation of Wnt/β-catenin signaling in cancer. Oncotarget 7, 17991-8005
26908451   Curated Info

6

Al Thawadi H, et al. (2016) VE-cadherin cleavage by ovarian cancer microparticles induces β-catenin phosphorylation in endothelial cells. Oncotarget 7, 5289-305
26700621   Curated Info

7

Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290
27362937   Curated Info

8

Malec V, Coulson JM, Urbé S, Clague MJ (2015) Combined Analyses of the VHL and Hypoxia Signaling Axes in an Isogenic Pairing of Renal Clear Cell Carcinoma Cells. J Proteome Res 14, 5263-72
26506913   Curated Info

9

Lemieux E, et al. (2015) Oncogenic KRAS signalling promotes the Wnt/β-catenin pathway through LRP6 in colorectal cancer. Oncogene 34, 4914-27
25500543   Curated Info

10

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

11

Llado V, et al. (2015) Repression of Intestinal Stem Cell Function and Tumorigenesis through Direct Phosphorylation of β-Catenin and Yap by PKCζ Cell Rep
25660024   Curated Info

12

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

13

Wang R, et al. (2014) Global discovery of high-NaCl-induced changes of protein phosphorylation. Am J Physiol Cell Physiol 307, C442-54
24965592   Curated Info

14

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

15

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

16

Flentke GR, Garic A, Hernandez M, Smith SM (2014) CaMKII represses transcriptionally active β-catenin to mediate acute ethanol neurodegeneration and can phosphorylate β-catenin. J Neurochem 128, 523-35
24117889   Curated Info

17

Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76
24117733   Curated Info

18

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

19

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

20

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

21

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
23836654   Curated Info

22

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

23

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

24

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

25

Verano-Braga T, et al. (2012) Time-resolved quantitative phosphoproteomics: new insights into Angiotensin-(1-7) signaling networks in human endothelial cells. J Proteome Res 11, 3370-81
22497526   Curated Info

26

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

27

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

28

Rey O, et al. (2012) Negative cross-talk between calcium-sensing receptor and β-catenin signaling systems in colonic epithelium. J Biol Chem 287, 1158-67
22094462   Curated Info

29

Guo A (2011) CST Curation Set: 11890; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

30

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

31

Possemato A (2010) CST Curation Set: 10868; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y/M)Xp[ST](L/I/M)
Curated Info

32

Lee G, et al. (2010) Phosphoinositide 3-kinase signaling mediates beta-catenin activation in intestinal epithelial stem and progenitor cells in colitis. Gastroenterology 139, 869-81, 881.e1-9
20580720   Curated Info

33

Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304
20686112   Curated Info

34

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

35

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
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36

Yamamura S, et al. (2010) Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer. Mol Cancer Ther 9, 1680-7
20501806   Curated Info

37

Nava P, et al. (2010) Interferon-gamma regulates intestinal epithelial homeostasis through converging beta-catenin signaling pathways. Immunity 32, 392-402
20303298   Curated Info

38

Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9
20166139   Curated Info

39

Raijmakers R, et al. (2010) Exploring the human leukocyte phosphoproteome using a microfluidic reversed-phase-TiO2-reversed-phase high-performance liquid chromatography phosphochip coupled to a quadrupole time-of-flight mass spectrometer. Anal Chem 82, 824-32
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40

Maher MT, et al. (2010) Beta-catenin phosphorylated at serine 45 is spatially uncoupled from beta-catenin phosphorylated in the GSK3 domain: implications for signaling. PLoS One 5, e10184
20419129   Curated Info

41

Van Hoof D, et al. (2009) Phosphorylation dynamics during early differentiation of human embryonic stem cells. Cell Stem Cell 5, 214-26
19664995   Curated Info

42

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

43

Zhou J (2009) CST Curation Set: 6346; Year: 2009; Biosample/Treatment: cell line, HeLa/UV; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

44

Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8
19276368   Curated Info

45

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

46

Moritz A (2008) CST Curation Set: 5781; Year: 2008; Biosample/Treatment: cell line, NCI-H3255/Iressa; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

47

Moritz A (2008) CST Curation Set: 5782; Year: 2008; Biosample/Treatment: cell line, NCI-H3255/Iressa; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

48

Moritz A (2008) CST Curation Set: 5777; Year: 2008; Biosample/Treatment: cell line, MKN-45/Su11274; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

49

Moritz A (2008) CST Curation Set: 5779; Year: 2008; Biosample/Treatment: cell line, MKN-45/Su11274; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

50

Thingholm TE, et al. (2008) TiO2-Based Phosphoproteomic Analysis of the Plasma Membrane and the Effects of Phosphatase Inhibitor Treatment. J Proteome Res 7, 3304-3313
18578522   Curated Info

51

Cantin GT, et al. (2008) Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. J Proteome Res 7, 1346-51
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52

Fang D, et al. (2007) Phosphorylation of beta-catenin by AKT promotes beta-catenin transcriptional activity. J Biol Chem 282, 11221-9
17287208   Curated Info

53

Taurin S, et al. (2006) Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinase. J Biol Chem 281, 9971-6
16476742   Curated Info