Ser170
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Home > Phosphorylation Site Page: > Ser170  -  NFAT3 (mouse)

Site Information
GGAFFsPsPGSSSLS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448860

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mutation of modification site ( 4 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 ) , western blotting ( 1 , 2 , 3 , 4 )
Relevant cell line - cell type - tissue:
3T3-L1 (fibroblast) ( 5 ) , adipose tissue [ERK5 (mouse), homozygous knockout] ( 2 ) , MEF (fibroblast) ( 3 , 4 ) , neuron-'brain, cerebellum' ( 1 )

Upstream Regulation
Regulatory protein:
ERK5 (mouse) ( 2 ) , IRAK1 (mouse) ( 3 )
Putative in vivo kinases:
ERK5 (mouse) ( 2 , 4 ) , mTOR (mouse) ( 4 ) , P38A (mouse) ( 4 )
Kinases, in vitro:
ERK5 (human) ( 4 ) , mTOR (human) ( 4 ) , P38A (human) ( 4 )
Treatments:
calcineurin ( 1 ) , calyculin_A ( 4 ) , ciclosporin ( 4 ) , FK520 ( 4 ) , HNMPA(AM)3 ( 1 ) , IL-18 ( 5 ) , ionomycin ( 4 ) , ionomycin_withdrawal ( 4 ) , isoproterenol ( 2 ) , neuritin ( 1 ) , okadaic_acid ( 4 ) , Pam3CSK4 ( 3 ) , PD98059 ( 4 ) , rapamycin ( 4 ) , SB203580 ( 4 ) , U0126 ( 4 ) , UV ( 4 ) , wortmannin ( 4 )

Downstream Regulation
Effects of modification on NFAT3:
activity, inhibited ( 3 ) , intracellular localization ( 1 )
Effects of modification on biological processes:
transcription, altered ( 3 , 4 )

References 

1

Yao JJ, et al. (2016) Neuritin Up-regulates Kv4.2 α-Subunit of Potassium Channel Expression and Affects Neuronal Excitability by Regulating the Calcium-Calcineurin-NFATc4 Signaling Pathway. J Biol Chem 291, 17369-81
27307045   Curated Info

2

Zhu H, et al. (2014) Role of Extracellular Signal-regulated Kinase 5 in Adipocyte Signaling. J Biol Chem 289, 6311-22
24425864   Curated Info

3

Wang D, Fasciano S, Li L (2008) The interleukin-1 receptor associated kinase 1 contributes to the regulation of NFAT. Mol Immunol 45, 3902-8
18691762   Curated Info

4

Yang TT, et al. (2008) Integration of protein kinases mTOR and extracellular signal-regulated kinase 5 in regulating nucleocytoplasmic localization of NFATc4. Mol Cell Biol 28, 3489-501
18347059   Curated Info

5

Chandrasekar B, et al. (2008) Interleukin-18 Suppresses Adiponectin Expression in 3T3-L1 Adipocytes via a Novel Signal Transduction Pathway Involving ERK1/2-dependent NFATc4 Phosphorylation. J Biol Chem 283, 4200-9
18086672   Curated Info