Ser279
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Home > Phosphorylation Site Page: > Ser279  -  GJA1 (human)

Site Information
sSPtAPLsPMsPPGy   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448853

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 5 , 10 , 11 , 16 ) , mutation of modification site ( 4 ) , phospho-antibody ( 7 , 8 , 13 , 14 , 15 , 17 , 18 ) , western blotting ( 4 , 7 , 8 , 14 , 15 )
Disease tissue studied:
breast cancer ( 5 , 11 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , cervical cancer ( 17 ) , lung cancer ( 11 ) , non-small cell lung cancer ( 11 ) , neuroblastoma ( 10 )
Relevant cell line - cell type - tissue:
astrocyte ( 14 ) , breast ( 1 ) , BT-549 (breast cell) ( 11 ) , Calu 6 (pulmonary) ( 11 ) , cervix ( 17 ) , CHO (fibroblast) ( 15 ) , E14tg2a ('stem, embryonic') ( 7 ) , H2009 (pulmonary) ( 11 ) , H2077 (pulmonary) ( 11 ) , H2887 (pulmonary) ( 11 ) , H322M (pulmonary) ( 11 ) , HeLa (cervical) ( 4 ) , HMLER ('stem, breast cancer') ( 5 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 5 ) , IMR32 (neural crest) ( 18 ) , Jurkat (T lymphocyte) ( 16 ) , keratinocyte-skin ( 13 ) , LOU-NH91 (squamous) ( 11 ) , MDA-MB-231 (breast cell) ( 11 ) , MDCK (epithelial) ( 15 ) , NB10 (neural crest) ( 10 ) , NCI-H1648 (pulmonary) ( 11 ) , NCI-H460 (pulmonary) ( 11 ) , NPC (neural crest) ( 10 )

Upstream Regulation
Putative in vivo kinases:
P38A (human) ( 13 )
Treatments:
cAMP_analog ( 12 ) , CXCL12 ( 5 ) , EGF ( 4 , 7 ) , phorbol_ester ( 8 , 15 ) , pressure ( 14 ) , SB202190 ( 13 ) , SB203580 ( 13 ) , U0126 ( 14 ) , UV ( 13 )

Downstream Regulation
Effects of modification on GJA1:
intracellular localization ( 17 ) , molecular association, regulation ( 7 ) , protein degradation ( 4 , 13 ) , ubiquitination ( 4 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 13 ) , endocytosis, induced ( 7 )
Induce interaction with:
CLTA (human) ( 7 )

Disease / Diagnostics Relevance
Relevant diseases:
cervical cancer ( 17 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Boeldt DS, et al. (2015) Phosphorylation of Ser-279/282 and Tyr-265 positions on Cx43 as possible mediators of VEGF-165 inhibition of pregnancy-adapted Ca2+ burst function in ovine uterine artery endothelial cells. Mol Cell Endocrinol 412, 73-84
26033246   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Schmitt M, et al. (2014) Mutation of human connexin43 amino acids s279/s282 increases protein stability upon treatment with epidermal growth factor. Cell Biochem Biophys 69, 379-84
24399133   Curated Info

5

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

6

Forster T, et al. (2014) Sulforaphane counteracts aggressiveness of pancreatic cancer driven by dysregulated Cx43-mediated gap junctional intercellular communication. Oncotarget 5, 1621-34
24742583   Curated Info

7

Fong JT, Nimlamool W, Falk MM (2014) EGF induces efficient Cx43 gap junction endocytosis in mouse embryonic stem cell colonies via phosphorylation of Ser262, Ser279/282, and Ser368. FEBS Lett 588, 836-44
24492000   Curated Info

8

Dunn CA, Lampe PD (2014) Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size. J Cell Sci 127, 455-64
24213533   Curated Info

9

Grosely R, et al. (2013) Effects of Phosphorylation on the Structure and Backbone Dynamics of the Intrinsically Disordered Connexin43 C-terminal Domain. J Biol Chem 288, 24857-70
23828237   Curated Info

10

DeNardo BD, et al. (2013) Quantitative phosphoproteomic analysis identifies activation of the RET and IGF-1R/IR signaling pathways in neuroblastoma. PLoS One 8, e82513
24349301   Curated Info

11

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

12

Joshi CN, et al. (2012) Control of vascular smooth muscle cell growth by connexin 43. Front Physiol 3, 220
22737133   Curated Info

13

Bellei B, et al. (2008) Ultraviolet A induced modulation of gap junctional intercellular communication by P38 MAPK activation in human keratinocytes. Exp Dermatol 17, 115-24
18047584   Curated Info

14

Malone P, et al. (2007) Pressure induces loss of gap junction communication and redistribution of connexin 43 in astrocytes. Glia 55, 1085-98
17551925   Curated Info

15

Solan JL, Lampe PD (2007) Key connexin 43 phosphorylation events regulate the gap junction life cycle. J Membr Biol 217, 35-41
17629739   Curated Info

16

Possemato A (2007) CST Curation Set: 2928; Year: 2007; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

17

Steinhoff I, et al. (2006) Phosphorylation of the gap junction protein Connexin43 in CIN III lesions and cervical carcinomas. Cancer Lett 235, 291-7
15958277   Curated Info

18

Arnold JM, Phipps MW, Chen J, Phipps J (2005) Cellular sublocalization of Cx43 and the establishment of functional coupling in IMR-32 neuroblastoma cells. Mol Carcinog 42, 159-69
15605363   Curated Info