Ser255

Site Information
HAtTGPLsPskDCGs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448852

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 3 , 4 , 6 , 7 , 9 , 11 ) , mass spectrometry (in vitro) ( 10 ) , mutation of modification site ( 1 , 8 ) , phospho-antibody ( 2 , 5 , 12 ) , western blotting ( 2 , 5 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 3 ) , brain ( 1 ) , HaCaT (keratinocyte) ( 5 ) , heart ( 6 , 11 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 4 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 4 ) , HL-1 (myocyte) ( 4 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 9 ) , MEF (fibroblast) ( 7 ) , oocyte ( 8 ) , ovary ( 8 , 12 ) , TM4 (sertoli) ( 2 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 3 , 4 , 6 , 7 , 9 , 11 ) , mass spectrometry (in vitro) ( 10 ) , mutation of modification site ( 1 , 8 ) , phospho-antibody ( 2 , 5 , 12 ) , western blotting ( 2 , 5 )

Relevant cell line - cell type - tissue:

'3T3-L1, differentiated' (adipocyte) ( 3 ) , brain ( 1 ) , HaCaT (keratinocyte) ( 5 ) , heart ( 6 , 11 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 4 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 4 ) , HL-1 (myocyte) ( 4 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 9 ) , MEF (fibroblast) ( 7 ) , oocyte ( 8 ) , ovary ( 8 , 12 ) , TM4 (sertoli) ( 2 )

Upstream Regulation
Kinases, in vitro:	ERK2 (mouse) ( 10 )
Treatments:	benzo(a)pyrene ( 2 ) , LH ( 12 ) , phorbol_ester ( 5 ) , U0124 ( 12 ) , U0126 ( 12 )

Downstream Regulation
Effects of modification on GJA1:	protein degradation ( 5 ) , ubiquitination ( 5 )
Effects of modification on biological processes:	endocytosis, induced ( 5 )

References
1	Freitas-Andrade M, et al. (2019) Targeting MAPK phosphorylation of Connexin43 provides neuroprotection in stroke. J Exp Med 216, 916-935 30872361 Curated Info
2	Ji X, et al. (2018) Benzo[a]pyrene-decreased gap junctional intercellular communication via calcium/calmodulin signaling increases apoptosis in TM4 cells. J Appl Toxicol 29578247 Curated Info
3	Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6 26060331 Curated Info
4	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
5	Smyth JW, et al. (2014) A 14-3-3 mode-1 binding motif initiates gap junction internalization during acute cardiac ischemia. Traffic 15, 684-99 24612377 Curated Info
6	Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11 23737553 Curated Info
7	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
8	Dyce PW, Norris RP, Lampe PD, Kidder GM (2012) Phosphorylation of Serine Residues in the C-terminal Cytoplasmic Tail of Connexin43 Regulates Proliferation of Ovarian Granulosa Cells. J Membr Biol 245, 291-301 22729691 Curated Info
9	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
10	Carlson SM, et al. (2011) Large-Scale Discovery of ERK2 Substrates Identifies ERK-Mediated Transcriptional Regulation by ETV3. Sci Signal 4, rs11 22028470 Curated Info
11	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
12	Norris RP, et al. (2008) Luteinizing hormone causes MAP kinase-dependent phosphorylation and closure of connexin 43 gap junctions in mouse ovarian follicles: one of two paths to meiotic resumption. Development 135, 3229-38 18776144 Curated Info