Ser255
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Home > Phosphorylation Site Page: > Ser255  -  GJA1 (human)

Site Information
HAtsGALsPAkDCGs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448852

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 ) , phospho-antibody ( 2 , 3 , 5 , 17 , 18 ) , western blotting ( 2 , 3 , 5 , 17 )
Disease tissue studied:
atrial fibrillation ( 16 ) , breast cancer ( 8 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , lung cancer ( 8 ) , non-small cell lung cancer ( 8 )
Relevant cell line - cell type - tissue:
'stem, embryonic' ( 14 ) , breast ( 1 ) , BT-549 (breast cell) ( 8 ) , E14tg2a ('stem, embryonic') ( 5 ) , Flp-In T-Rex-293 (epithelial) ( 9 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 9 ) , H2077 (pulmonary) ( 8 ) , H322 (pulmonary) ( 8 ) , H322M (pulmonary) ( 8 ) , heart ( 16 , 17 ) , HEGCs ( 3 ) , HeLa (cervical) ( 11 , 15 ) , HES-3 ('stem, embryonic') ( 12 ) , HESCs (endometrial) ( 3 ) , HMEC (endothelial) ( 2 ) , HOP62 (pulmonary) ( 8 ) , HUES-9 ('stem, embryonic') ( 13 ) , IMR32 (neural crest) ( 18 ) , MDA-MB-231 (breast cell) ( 8 ) , NCI-H1395 (pulmonary) ( 8 ) , NCI-H2030 (pulmonary) ( 8 )

Upstream Regulation
Regulatory protein:
HSP70 (human) ( 2 )
Treatments:
cAMP_analog ( 10 ) , colforsin ( 18 ) , EGF ( 5 ) , levonorgestrel/norgestrel ( 3 ) , phorbol_ester ( 18 )

Downstream Regulation
Effects of modification on GJA1:
intracellular localization ( 3 , 18 ) , molecular association, regulation ( 5 )
Effects of modification on biological processes:
endocytosis, induced ( 5 )
Induce interaction with:
CLTA (human) ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
AC ( 17 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Thuringer D, et al. (2015) Oncogenic extracellular HSP70 disrupts the gap-junctional coupling between capillary cells. Oncotarget 6, 10267-83
25868858   Curated Info

3

Zhao X, et al. (2015) Levonorgestrel Inhibits Human Endometrial Cell Proliferation through the Upregulation of Gap Junctional Intercellular Communication via the Nuclear Translocation of Ser255 Phosphorylated Cx43. Biomed Res Int 2015, 758684
26161412   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Fong JT, Nimlamool W, Falk MM (2014) EGF induces efficient Cx43 gap junction endocytosis in mouse embryonic stem cell colonies via phosphorylation of Ser262, Ser279/282, and Ser368. FEBS Lett 588, 836-44
24492000   Curated Info

6

Grosely R, et al. (2013) Effects of Phosphorylation on the Structure and Backbone Dynamics of the Intrinsically Disordered Connexin43 C-terminal Domain. J Biol Chem 288, 24857-70
23828237   Curated Info

7

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

8

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

9

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

10

Joshi CN, et al. (2012) Control of vascular smooth muscle cell growth by connexin 43. Front Physiol 3, 220
22737133   Curated Info

11

Grosstessner-Hain K, et al. (2011) Quantitative phospho-proteomics to investigate the polo-like kinase 1-dependent phospho-proteome. Mol Cell Proteomics 10, M111.008540
21857030   Curated Info

12

Ding VM, et al. (2011) Tyrosine phosphorylation profiling in fgf-2 stimulated human embryonic stem cells. PLoS One 6, e17538
21437283   Curated Info

13

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

14

Brill LM, et al. (2009) Phosphoproteomic analysis of human embryonic stem cells. Cell Stem Cell 5, 204-13
19664994   Curated Info

15

Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8
19276368   Curated Info

16

Stokes M (2008) CST Curation Set: 4145; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

17

Sato T, et al. (2008) Altered expression of connexin43 contributes to the arrhythmogenic substrate during the development of heart failure in cardiomyopathic hamster. Am J Physiol Heart Circ Physiol 294, H1164-73
18065522   Curated Info

18

Arnold JM, Phipps MW, Chen J, Phipps J (2005) Cellular sublocalization of Cx43 and the establishment of functional coupling in IMR-32 neuroblastoma cells. Mol Carcinog 42, 159-69
15605363   Curated Info