Ser255
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Home > Phosphorylation Site Page: > Ser255  -  GJA1 (rat)

Site Information
HATTGPLsPskDCGs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448852

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 , 8 ) , electrophoretic mobility shift ( 6 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 5 ) , mass spectrometry (in vitro) ( 3 ) , mutation of modification site ( 1 , 4 , 6 , 7 ) , phosphoamino acid analysis ( 7 ) , phosphopeptide mapping ( 8 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) [GJA1 (rat)] ( 6 ) , 293 (epithelial) [MEK5 (human)] ( 6 ) , fibroblast [GJA1 (mouse)] ( 4 ) , heart ( 5 ) , HeLa (cervical) ( 1 ) , MDCK (epithelial) ( 1 ) , T51B (epithelial) ( 8 )

Upstream Regulation
Putative in vivo kinases:
ERK5 (human) ( 6 )
Kinases, in vitro:
CAMK2A (human) ( 3 ) , ERK1 (rat) ( 8 ) , ERK2 (mouse) ( 7 ) , ERK5 (human) ( 6 )
Treatments:
EGF ( 7 ) , LPA ( 7 ) , PD98059 ( 7 ) , vanadate ( 7 )

Downstream Regulation
Effects of modification on GJA1:
activity, inhibited ( 7 )

References 

1

Thévenin AF, et al. (2017) Phosphorylation regulates connexin43/ZO-1 binding and release, an important step in gap junction turnover. Mol Biol Cell 28, 3595-3608
29021339   Curated Info

2

Mitra SS, Xu J, Nicholson BJ (2012) Coregulation of Multiple Signaling Mechanisms in pp60v-Src-Induced Closure of Cx43 Gap Junction Channels. J Membr Biol 245, 495-506
22965738   Curated Info

3

Huang RY, et al. (2011) Identification of CaMKII phosphorylation sites in Connexin43 by high-resolution mass spectrometry. J Proteome Res 10, 1098-109
21158428   Curated Info

4

Lin R, et al. (2006) v-Src tyrosine phosphorylation of connexin43: regulation of gap junction communication and effects on cell transformation. Cell Commun Adhes 13, 199-216
16916748   Curated Info

5

Axelsen LN, et al. (2006) Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123). J Mol Cell Cardiol 40, 790-8
16678851   Curated Info

6

Cameron SJ, et al. (2003) Regulation of epidermal growth factor-induced connexin 43 gap junction communication by big mitogen-activated protein kinase1/ERK5 but not ERK1/2 kinase activation. J Biol Chem 278, 18682-8
12637502   Curated Info

7

Warn-Cramer BJ, Cottrell GT, Burt JM, Lau AF (1998) Regulation of connexin-43 gap junctional intercellular communication by mitogen-activated protein kinase. J Biol Chem 273, 9188-96
9535909   Curated Info

8

Warn-Cramer BJ, et al. (1996) Characterization of the mitogen-activated protein kinase phosphorylation sites on the connexin-43 gap junction protein. J Biol Chem 271, 3779-86
8631994   Curated Info