Ser649
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Home > Phosphorylation Site Page: > Ser649  -  TIF1A (human)

Site Information
PVLYMQPsPL_____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448839

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , immunoprecipitation ( 8 , 9 ) , mass spectrometry ( 1 , 2 , 5 , 6 ) , mutation of modification site ( 7 , 8 , 9 , 10 ) , phosphopeptide mapping ( 10 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , leukemia ( 5 ) , acute myelogenous leukemia ( 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
RSK2 (human) ( 10 )
Treatments:
PD98059 ( 10 )

Downstream Regulation
Effects of modification on TIF1A:
activity, induced ( 10 ) , molecular association, regulation ( 8 , 9 )
Effects of modification on biological processes:
cell cycle regulation ( 8 ) , cell growth, induced ( 7 ) , transcription, induced ( 7 , 8 )
Induce interaction with:
POLR1B (human) ( 9 ) , POLR2F (human) ( 8 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

5

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

6

Guo A (2011) CST Curation Set: 12068; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

7

Gomes C, et al. (2011) RNA polymerase 1-driven transcription as a mediator of BDNF-induced neurite outgrowth. J Biol Chem 286, 4357-63
21098478   Curated Info

8

Gorski SA, et al. (2008) Modulation of RNA polymerase assembly dynamics in transcriptional regulation. Mol Cell 30, 486-97
18498750   Curated Info

9

Philimonenko VV, et al. (2004) Nuclear actin and myosin I are required for RNA polymerase I transcription. Nat Cell Biol 6, 1165-72
15558034   Curated Info

10

Zhao J, Yuan X, Frödin M, Grummt I (2003) ERK-dependent phosphorylation of the transcription initiation factor TIF-IA is required for RNA polymerase I transcription and cell growth. Mol Cell 11, 405-13
12620228   Curated Info